| Title: |
Bovine islets are less susceptible than human islets to damage by human cytokines. |
| Authors: |
Piro S.; Lupi R.; Patanè G.; Rabuazzo M.A.; Marselli L.; Santangelo C.; Realacci M.; Del Guerra S.; Purrello F.; Marchetti P.; DOTTA, FRANCESCO |
| Contributors: |
Piro, S.; Lupi, R.; Dotta, Francesco; Patanè, G.; Rabuazzo, M. A.; Marselli, L.; Santangelo, C.; Realacci, M.; Del Guerra, S.; Purrello, F.; Marchetti, P. |
| Publication Year: |
2001 |
| Collection: |
Università degli Studi di Siena: USiena air |
| Description: |
BACKGROUND: The potential benefits of islet xenografting in type 1 diabetes include the intriguing, but still unanswered, possibility that the grafted xenoislets may be less subjected to human autoimmune attack. Cytokines may play a major role in the pathogenesis of autoimmune diabetes by causing impairment of insulin release and pancreatic islet cell toxicity. METHODS: We compared insulin secretion, islet cell death and survival, inducible nitric oxide synthase (iNOS) mRNA expression, nitrite production, and Bcl-2 and Bax mRNA expression in isolated human and large mammal (bovine) islets exposed to 50 U/ml recombinant human interleukin-1, 1,000 U/ml recombinant human tumor necrosis factor-alpha and 1,000 U/ml recombinant human interferon-gamma. RESULTS: After 24-hr exposure, a marked decrease of glucose-stimulated insulin secretion was observed with human, but not with bovine islets. After 48-hr exposure, human, but not bovine, pancreatic islets showed a significantly higher percentage of apoptotic cells compared to controls. Treatment of human islets with human cytokines induced up-regulation of iNOS mRNA, increased levels of nitrites, and down-regulation of Bcl-2 mRNA, with unchanged levels of Bax mRNA. These parameters were not affected by cytokines in bovine islets. CONCLUSIONS: Bovine islets are less susceptible than human islets to the effects of human cytokines, which may be a potential advantage of xenotransplantation. |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/11211190; info:eu-repo/semantics/altIdentifier/wos/WOS:000166637500004; volume:71; issue:1; firstpage:21; lastpage:26; numberofpages:6; journal:TRANSPLANTATION; http://hdl.handle.net/11365/33158 |
| DOI: |
10.1097/00007890-200101150-00004 |
| Availability: |
http://hdl.handle.net/11365/33158; https://doi.org/10.1097/00007890-200101150-00004 |
| Rights: |
info:eu-repo/semantics/closedAccess |
| Accession Number: |
edsbas.2764AE4A |
| Database: |
BASE |