| Title: |
Multicenter evaluation of rapid BACpro® II for the accurate identification of microorganisms directly from blood cultures using MALDI-TOF MS |
| Authors: |
Oviaño, Marina; Ingebretsen, André; Steffensen, Anne Katrine; Croxatto, Antony; Prod’hom, Guy; Quiroga, Lidia; Bou, Germán; Greub, Gilbert; Rodríguez-Temporal, David; Rodríguez-Sánchez, Belén |
| Source: |
2075-4418. |
| Publication Year: |
2022 |
| Collection: |
Universitet i Oslo: Digitale utgivelser ved UiO (DUO) |
| Description: |
The identification of microorganisms directly from blood cultures using MALDI-TOF MS has been shown to be the most impacting application of this methodology. In this study, a novel commercial method was evaluated in four clinical microbiology laboratories. Positive blood culture samples (n = 801) were processed using a rapid BACpro® II kit and then compared with the routine gold standard. A subset of monomicrobial BCs (n = 560) were analyzed in parallel with a Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) and compared with the rapid BACpro® II kit. In addition, this kit was also compared with two different in-house methods. Overall, 80.0% of the monomicrobial isolates (609/761; 95% CI 71.5–88.5) were correctly identified by the rapid BACpro® II kit at the species level (92.3% of the Gram negative and 72.4% of the Gram positive bacteria). The comparison with the Sepsityper® Kit showed that the rapid BACpro® II kit generated higher rates of correct species-level identification for all categories (p > 0.0001), except for yeasts identified with score values > 1.7. It also proved superior to the ammonium chloride method (p > 0.0001), but the differential centrifugation method allowed for higher rates of correct identification for Gram negative bacteria (p > 0.1). The percentage of accurate species-level identification of Gram positive bacteria was particularly noteworthy in comparison with other commercial and in-house methods. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
http://hdl.handle.net/10852/92901; 1975503; Diagnostics (Basel); 11; 12; https://doi.org/10.3390/diagnostics11122251 |
| DOI: |
10.3390/diagnostics11122251 |
| Availability: |
http://hdl.handle.net/10852/92901; http://urn.nb.no/URN:NBN:no-95474; https://doi.org/10.3390/diagnostics11122251 |
| Rights: |
Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.27B73C5 |
| Database: |
BASE |