| Title: |
Crosstalk between Helicobacter pylori and gastric epithelial cells is impaired by docosahexaenoic acid. |
| Authors: |
Correia, Marta; Michel, Valérie; Osório, Hugo; El Ghachi, Meriem; Bonis, Mathilde; Boneca, Ivo G; de Reuse, Hilde; Matos, António A; Lenormand, Pascal; Seruca, Raquel; Figueiredo, Ceu; Machado, Jose Carlos; Touati, Eliette |
| Contributors: |
Universidade do Porto = University of Porto; Pathogenèse de Helicobacter; Institut Pasteur Paris (IP); Biologie et Génétique de la Paroi bactérienne - Biology and Genetics of Bacterial Cell Wall (BGPB); Centro Hospitalar de Lisboa Central E.P.E; Protéomique (Plate-Forme); This study was supported by the ERA-NET Pathogenomics (ERA-PTG/0001/2010) and by the Portuguese Foundation for Science and Technology (FCT -PTDC/SAU-SAP/120024/2010). MC is supported by an FCT fellowship BD/36689/2007. The Acçoes integradas Luso-Francesas (CRUP-PAULIF) Portugal and Francealso provided financial support (AF-8/09). Mathilde Bonis was supported by a PhD fellowship (Ministère de l’Enseignement Supérieur et de la Recherche, France).This study was also supported by the ERC starting grant (PGNfromSHAPEtoVIR number 202283) to Ivo G. Bonec; European Project: 202283,ERC-2007-StG,ERC-2007-StG,PGNFROMSHAPETOVIR(2008); European Project: 116470,FCT::,ERA-PTG/2010,ERA-PTG/0001/2010(2011) |
| Source: |
ISSN: 1932-6203. |
| Publisher Information: |
CCSD; Public Library of Science |
| Publication Year: |
2013 |
| Collection: |
Institut Pasteur: HAL |
| Subject Terms: |
Helicobacter Infections/microbiology; Docosahexanoic acid DHA; Antibacterial factors; MESH: Anti-Inflammatory Agents; MESH: Bacterial Adhesion; MESH: Bacterial Outer Membrane Proteins; MESH: Cell Wall; MESH: Docosahexaenoic Acids; MESH: Epithelial Cells; MESH: Helicobacter pylori; MESH: Inflammation; MESH: Stomach; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| Description: |
International audience ; H. pylori colonizes half of the world's population leading to gastritis, ulcers and gastric cancer. H. pylori strains resistant to antibiotics are increasing which raises the need for alternative therapeutic approaches. Docosahexaenoic acid (DHA) has been shown to decrease H. pylori growth and its associated-inflammation through mechanisms poorly characterized. We aimed to explore DHA action on H. pylori-mediated inflammation and adhesion to gastric epithelial cells (AGS) and also to identify bacterial structures affected by DHA. H. pylori growth and metabolism was assessed in liquid cultures. Bacterial adhesion to AGS cells was visualized by transmission electron microscopy and quantified by an Enzyme Linked Immunosorbent Assay. Inflammatory proteins were assessed by immunoblotting in infected AGS cells, previously treated with DHA. Bacterial total and outer membrane protein composition was analyzed by 2-dimensional gel electrophoresis. Concentrations of 100 µM of DHA decreased H. pylori growth, whereas concentrations higher than 250 µM irreversibly inhibited bacteria survival. DHA reduced ATP production and adhesion to AGS cells. AGS cells infected with DHA pre-treated H. pylori showed a 3-fold reduction in Interleukin-8 (IL-8) production and a decrease of COX2 and iNOS. 2D electrophoresis analysis revealed that DHA changed the expression of H. pylori outer membrane proteins associated with stress response and metabolism and modified bacterial lipopolysaccharide phenotype. As conclusions our results show that DHA anti-H. pylori effects are associated with changes of bacteria morphology and metabolism, and with alteration of outer membrane proteins composition, that ultimately reduce the adhesion of bacteria and the burden of H. pylori-related inflammation. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/23577140; info:eu-repo/grantAgreement//202283/EU/The role of peptidoglycan in bacterial cell physiology: from bacterial shape to host-microbe interactions/PGNFROMSHAPETOVIR; info:eu-repo/grantAgreement/FCT//116470/EU/Helicobacter pylori diversity in pathogenesis, antibiotic resistance, and evasion from natural and vaccine-induced immune responses (HELDIVPAT)/ERA-PTG/0001/2010; PUBMED: 23577140; PUBMEDCENTRAL: PMC3618039 |
| DOI: |
10.1371/journal.pone.0060657 |
| Availability: |
https://pasteur.hal.science/pasteur-01441046; https://pasteur.hal.science/pasteur-01441046v1/document; https://pasteur.hal.science/pasteur-01441046v1/file/journal.pone.0060657.PDF; https://doi.org/10.1371/journal.pone.0060657 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.27E02F02 |
| Database: |
BASE |