| Title: |
2P-FENDO-II: a fiber bundle microscope for all optical brain study on large field of view in freely moving mice |
| Authors: |
Blot, François, G. C.; Decombe, Dimitri; Lorca-Cámara, Antonio; Anquetil, Maya; de Sars, Vincent; Tourain, Christophe; Forget, Benoît, C.; Accanto, Nicolò; Emiliani, Valentina |
| Contributors: |
Institut de la Vision; Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Norwegian University of Science and Technology = Norges Teknisk-Naturvitenskapelige Universitet = Norjan teknis-luonnontieteellinen yliopisto (NTNU); IHU FOReSIGHT grant (grant P-ALLOP3-IHU-000 and P-HOLO00-IHU-000); ANR-19-CE19-0001,2MEnHoloMD,Un double microscope/endoscope pour la manipulation par holographie des circuits visuels profonds(2019); ANR-23-ERCS-0009,2P-COMFIB,Le fibroscope composé à deux photons : une plateforme universelle pour étudier le cerveau à toutes les échelles spatiales et temporelles.(2023); European Project: 885090,ERC-2019-ADG,ERC-2019-ADG,HOLOVIS(2020); European Project: 101016787,H2020-ICT-2018-20,H2020-ICT-2020-2,DEEPER(2021) |
| Source: |
ISSN: 2667-2375. |
| Publisher Information: |
CCSD; Cell Press Elsevier |
| Publication Year: |
2025 |
| Subject Terms: |
all-optical neuronal circuit manipulation; two-photon optogenetic photostimulation; two-photon calcium imaging; computer-generated holography; holographic microendoscopy; freely-moving mice; fiber bundle; [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]; [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging; [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic |
| Description: |
International audience ; All-optical strategies enable to identify functional neuronal ensembles with calcium imaging and replay/alter their spatio-temporal activity with optogenetics to decipher their behavioral implications. We have previously developed the first fiber-coupled microscope enabling twophoton (2P) functional imaging and 2P holographic photostimulation with near-single cell resolution in freely moving mice, named 2P-FENDO. Here, we present a significantly optimized 2P-FENDO-II system that achieves a four times larger field of view, a more homogeneous light distribution across the field of view, both for imaging and photostimulation, while achieving better flexibility and thus optimal adaptation to the study of freely moving mice. We demonstrate the performance and versatility of 2P-FENDO-II in experiments targeting the somatosensory cortex, the visual cortex or the cerebellar cortex, in which we show concomitant calcium imaging with jGCaMP7s and optogenetic control with ChRmine. These enhancements establish 2P-FENDO-II as a groundbreaking tool for all-optical interrogation of neuronal circuits on large volume in naturalistic situations. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/grantAgreement//885090/EU/Holographic control of visual circuits/HOLOVIS; info:eu-repo/grantAgreement//101016787/EU/DEEP BRAIN PHOTONIC TOOLS FOR CELL-TYPE SPECIFIC TARGETING OF NEURAL DISEASES/DEEPER |
| Availability: |
https://hal.science/hal-05407257; https://hal.science/hal-05407257v1/document; https://hal.science/hal-05407257v1/file/Blot-Decombe%20et%20al.%20HAL.pdf |
| Rights: |
https://creativecommons.org/licenses/by-nc-nd/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.281A9875 |
| Database: |
BASE |