| Title: |
A protocol for detection of large chromosome variations in banana using Next Generation Sequencing |
| Authors: |
Catherine, Breton; Cenci, Alberto; Sardos, Julie; Rachel, Chase; Max, Ruas; Mathieu, Rouard; Nicolas, Roux |
| Contributors: |
Alliance Bioversity International-CIAT |
| Source: |
Efficient Screening Techniques to Identify Mutants with Fusarium wilt TR4 Resistance in Banana Protocols ; https://hal.archives-ouvertes.fr/hal-03414944 ; Efficient Screening Techniques to Identify Mutants with Fusarium wilt TR4 Resistance in Banana Protocols, 2022 |
| Publisher Information: |
HAL CCSD |
| Publication Year: |
2022 |
| Collection: |
Archive ouverte HAL (Hyper Article en Ligne, CCSD - Centre pour la Communication Scientifique Directe) |
| Subject Terms: |
aneuploidy; banana; chromosomal variation; Musa spp; somaclonal variants; genebanks; NGS; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Core activities of genebank operations include the preservation of germplasm identity and maintenance of genetic integrity. Some organisms such as banana are maintained by tissue culture that can foster accumulation of somatic mutations and loss of genetic integrity. Such changes can be reflected in their genome structure and thus be revealed by sequencing methods. Here, we propose a protocol for the detection of large chromosomal gains and/or losses that was applied to in vitro banana accessions with dif ferent levels of ploidy. Mixoploidy was detected in triploid (3x) accessions with chromosomal regions being diploid (2x) and tetraploid (4x) and in diploid accessions (2x) where large deletions resulted in partial haploidy (1x). Such abnormal molecular karyotypes can potentially explain phenotypic aberrations observed in off type material. With the af fordable cost of Next Generation Sequencing (NGS) technologies and the release of the presented bioinformatic pipeline, we aim to promote the application of this methodology as a routine operation for genebank management as an important step to monitor the genetic integrity of distributed material. Moreover, genebank users can be also empowered to apply the methodology and check the molecular karyotype of the ordered material. |
| Document Type: |
book part |
| Language: |
English |
| Relation: |
hal-03414944; https://hal.archives-ouvertes.fr/hal-03414944; https://hal.archives-ouvertes.fr/hal-03414944/document; https://hal.archives-ouvertes.fr/hal-03414944/file/Breton_26102021.pdf |
| Availability: |
https://hal.archives-ouvertes.fr/hal-03414944; https://hal.archives-ouvertes.fr/hal-03414944/document; https://hal.archives-ouvertes.fr/hal-03414944/file/Breton_26102021.pdf |
| Accession Number: |
edsbas.287625B6 |
| Database: |
BASE |