| Title: |
Role of gangliosides in the association of ErbB2 with lipid rafts in mammary epithelial HC11 cells |
| Authors: |
E. Sottocornola; R. Misasi; V. Mattei; L. Ciarlo; R. Gradini; T. Garofalo; B. Berra; I. Colombo; M. Sorice |
| Contributors: |
E. Sottocornola; R. Misasi; V. Mattei; L. Ciarlo; R. Gradini; T. Garofalo; B. Berra; I. Colombo; M. Sorice |
| Publisher Information: |
Blackwell |
| Publication Year: |
2006 |
| Collection: |
The University of Milan: Archivio Istituzionale della Ricerca (AIR) |
| Subject Terms: |
epidermal growth factor receptor; ErbB2; GM3; HC11 cells; lipid rafts; Settore BIO/10 - Biochimica; Settore BIOS-07/A - Biochimica |
| Description: |
We analyzed the role of gangliosides in the association of the ErbB2 receptor tyrosine-kinase (RTK) with lipid rafts in mammary epithelial HC11 cells. Scanning confocal microscopy experiments revealed a strict ErbB2– GM3 colocalization in wild-type cells. In addition, analysis of membrane fractions obtained using a linear sucrose gradient showed that ErbB2, epidermal growth factor receptor (EGFR) and Shc-p66 (proteins correlated with the ErbB2 signal transduction pathway) were preferentially enriched in lipid rafts together with gangliosides. Blocking of endogenous ganglioside synthesis by (+⁄ –)-threo-1-phenyl-2-decanoylamino-3-morpholino- 1-propanol hydrochloride ([D]-PDMP) induced a drastic cell-surface redistribution of ErbB2, EGFR and Shc-p66, within the Triton-soluble fractions, as revealed by linear sucrose-gradient analysis. This redistribution was partially reverted when exogenous GM3 was added to gangliosidedepleted HC11 cells. The results point out the key role of ganglioside GM3 in retaining ErbB2 and signal-transduction-correlated proteins in lipid rafts. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/16623716; info:eu-repo/semantics/altIdentifier/wos/WOS:000236394100021; volume:273; issue:8; firstpage:1821; lastpage:1830; numberofpages:10; journal:THE FEBS JOURNAL; https://hdl.handle.net/2434/43099 |
| DOI: |
10.1111/j.1742-4658.2006.05203.x |
| Availability: |
https://hdl.handle.net/2434/43099; https://doi.org/10.1111/j.1742-4658.2006.05203.x |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by-sa/4.0/ |
| Accession Number: |
edsbas.28C94F88 |
| Database: |
BASE |