| Title: |
Two Isoforms of the mRNA Binding Protein IGF2BP2 Are Generated by Alternative Translational Initiation |
| Authors: |
Hang T. T. Le; Alice M. Sorrell; Kenneth Siddle |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
ftp://ftp.ncbi.nlm.nih.gov/pub/pmc/9b/97/PLoS_One_2012_Mar_12_7(3)_e33140.tar.gz |
| Publication Year: |
2011 |
| Collection: |
CiteSeerX |
| Description: |
IGF2BP2 is a member of a family of mRNA binding proteins that, collectively, have been shown to bind to several different mRNAs in mammalian cells, including one of the mRNAs encoding insulin-like growth factor-2. Polymorphisms in the Igf2bp2 gene are associated with risk of developing type 2 diabetes, but detailed functional characterisation of IGF2BP2 protein is lacking. By immunoblotting with C-terminally reactive antibodies we identified a novel IGF2BP2 isoform with a molecular weight of 58 kDa in both human and rodents, that is expressed at somewhat lower levels than the full-length 65 kDa protein. We demonstrated by mutagenesis that this isoform is generated by alternative translation initiation at the internal Met69. It lacks a conserved N-terminal RNA Recognition Motif (RRM) and would be predicted to differ functionally from the canonical full length isoform. We further investigated IGF2BP2 mRNA transcripts by amplification of cDNA using 59-RACE. We identified multiple transcription start sites of the human, mouse and rat Igf2bp2 genes in a highly conserved region only 50–90 nts upstream of the major translation start site, ruling out the existence of N-terminally extended isoforms. We conclude that structural heterogeneity of IGF2BP2 protein should be taken into account when considering |
| Document Type: |
text |
| File Description: |
application/zip |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.9962 |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.271.9962 |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.2917B01F |
| Database: |
BASE |