| Title: |
Nutlin 3a aa Improving the Bioactivity of a p53 MDM2 Interaction Inhibitor by Introducing a Solvent Exposed Methylene Group |
| Authors: |
Nietzold, F.; Rubner, S.; Labuzek, B.; Golik, P.; Surmiak, E.; Del Corte, X.; Kitel, R.; Protzel, C.; Reppich Sacher, R.; Stichel, J.; Magiera Mularz, K.; Holak, T.A.; Berg, T. |
| Source: |
ChemBioChem 24 2023 , p. e202300006 1 6 |
| Publication Year: |
2023 |
| Collection: |
Helmholtz Zentrum Berlin (HZB): Publications |
| Subject Terms: |
biological activity; drug design; inhibitors; protein structures; protein protein interactions |
| Description: |
Nutlin 3a is a reversible inhibitor of the p53 MDM2 interaction. We have synthesized the derivative Nutlin 3a aa bearing an additional exocyclic methylene group in the piperazinone moiety. Nutlin 3a aa is more active than Nutlin 3a against purified wild type MDM2, and is more effective at increasing p53 levels and releasing transcription of p53 target genes from MDM2 induced repression. X ray analysis of wild type MDM2 bound Nutlin 3a aa indicated that the orientation of its modified piperazinone ring was altered in comparison to the piperazinone ring of MDM2 bound Nutlin 3a, with the exocyclic methylene group of Nutlin 3a aa pointing away from the protein surface. Our data point to the introduction of exocyclic methylene groups as a useful approach by which to tailor the conformation of bioactive molecules for improved biological activity |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Availability: |
https://www.helmholtz-berlin.de/pubbin/oai_publication?VT=1&ID=109346 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.29552048 |
| Database: |
BASE |