| Title: |
The cellular architecture of the antimicrobial response network in human leprosy granulomas |
| Authors: |
Ma, Feiyang; Hughes, Travis K; Teles, Rosane MB; Andrade, Priscila R; de Andrade Silva, Bruno J; Plazyo, Olesya; Tsoi, Lam C; Do, Tran; Wadsworth, Marc H; Oulee, Aislyn; Ochoa, Maria Teresa; Sarno, Euzenir N; Iruela-Arispe, M Luisa; Klechevsky, Eynav; Bryson, Bryan; Shalek, Alex K; Bloom, Barry R; Gudjonsson, Johann E; Pellegrini, Matteo; Modlin, Robert L |
| Contributors: |
Massachusetts Institute of Technology. Department of Biological Engineering |
| Source: |
PMC |
| Publisher Information: |
Springer Science and Business Media LLC |
| Publication Year: |
2023 |
| Collection: |
DSpace@MIT (Massachusetts Institute of Technology) |
| Description: |
Granulomas are complex cellular structures composed predominantly of macrophages and lymphocytes that function to contain and kill invading pathogens. Here, we investigated the single-cell phenotypes associated with antimicrobial responses in human leprosy granulomas by applying single-cell and spatial sequencing to leprosy biopsy specimens. We focused on reversal reactions (RRs), a dynamic process whereby some patients with disseminated lepromatous leprosy (L-lep) transition toward self-limiting tuberculoid leprosy (T-lep), mounting effective antimicrobial responses. We identified a set of genes encoding proteins involved in antimicrobial responses that are differentially expressed in RR versus L-lep lesions and regulated by interferon-γ and interleukin-1β. By integrating the spatial coordinates of the key cell types and antimicrobial gene expression in RR and T-lep lesions, we constructed a map revealing the organized architecture of granulomas depicting compositional and functional layers by which macrophages, T cells, keratinocytes and fibroblasts can each contribute to the antimicrobial response. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Nature Immunology; https://hdl.handle.net/1721.1/147779; Ma, Feiyang, Hughes, Travis K, Teles, Rosane MB, Andrade, Priscila R, de Andrade Silva, Bruno J et al. 2021. "The cellular architecture of the antimicrobial response network in human leprosy granulomas." Nature Immunology, 22 (7). |
| Availability: |
https://hdl.handle.net/1721.1/147779 |
| Rights: |
Creative Commons Attribution-Noncommercial-Share Alike ; http://creativecommons.org/licenses/by-nc-sa/4.0/ |
| Accession Number: |
edsbas.296F3B10 |
| Database: |
BASE |