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WNT7B drives a program for pancreatic cancer subtype switching and progression

Title: WNT7B drives a program for pancreatic cancer subtype switching and progression
Authors: Sprangers, Joep; Bugter, Jeroen M.; Xanthakis, Despina; Boekhout, Michiel; Kok, Rutger N.U.; Geurts,Veerle E.; Clevers, Hans; Brosens, Lodewijk A.A.; Dijk,Frederike; Rodríguez Colman, Maria J.; van der Vaart, Jelte Y.; Maurice, Madelon M.; CMM; Cancer; CMM Groep Maurice; CMM Groep Rodriguez Colman; Hubrecht Institute with UMC; Pathologie Pathologen staf; Regenerative Medicine and Stem Cells
Publication Year: 2026
Subject Terms: cancer; cell biology; functional aspects of cell biology; General
Description: Hyperactivation of WNT signaling is a hallmark of cancer, often driven by increased expression of WNT ligands. In pancreatic ductal adenocarcinoma (PDAC), elevated WNT7B and WNT10A correlate with aggressive, basal-like disease and poor patient survival, but the mechanisms underlying this association remain unclear. Using patient-derived organoids, we show that WNT7B promotes proliferation and maintains basal-like transcriptional states by preventing differentiation toward a more classical PDAC signature. Clonal WNT7B reporter organoids reveal that WNT-high cells are heterogeneously distributed and stably coexist with WNT-low/negative lineages. Hybrid co-cultures demonstrate that WNT7B-expressing cells support the survival and growth of neighboring WNT-negative cells via short-range, contact-dependent signaling. These findings highlight the functional importance of heterogeneous WNT7B/10A expression in driving PDAC aggressiveness and suggest that targeted WNT inhibition may shift tumors toward a more differentiated, less aggressive state, offering potential therapeutic benefit.
Document Type: article in journal/newspaper
File Description: application/pdf
Language: English
ISSN: 2589-0042
Relation: https://dspace.library.uu.nl/handle/1874/469553
Availability: https://dspace.library.uu.nl/handle/1874/469553
Rights: info:eu-repo/semantics/OpenAccess
Accession Number: edsbas.29B4B15C
Database: BASE