| Title: |
Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density. |
| Authors: |
Gilfillan, Connie B; Wang, Chensu; Mohsen, Mona O.; Rufer, Nathalie; Hebeisen, Michael; Allard, Mathilde; Verdeil, Grégory; Irvine, Darrell J; Bachmann, Martin F.; Speiser, Daniel E |
| Source: |
Gilfillan, Connie B; Wang, Chensu; Mohsen, Mona O.; Rufer, Nathalie; Hebeisen, Michael; Allard, Mathilde; Verdeil, Grégory; Irvine, Darrell J; Bachmann, Martin F.; Speiser, Daniel E (2020). Murine CD8 T-cell functional avidity is stable in vivo but not in vitro: Independence from homologous prime/boost time interval and antigen density. European journal of immunology, 50(4), pp. 505-514. Wiley-VCH 10.1002/eji.201948355 |
| Publisher Information: |
Wiley-VCH |
| Publication Year: |
2020 |
| Collection: |
BORIS (Bern Open Repository and Information System, University of Bern) |
| Subject Terms: |
610 Medicine & health |
| Description: |
It is known that for achieving high affinity antibody responses, vaccines must be optimized for antigen dose/density, and the prime/boost interval should be at least 4 weeks. Similar knowledge is lacking for generating high avidity T-cell responses. The functional avidity (FA) of T cells, describing responsiveness to peptide, is associated with the quality of effector function and the protective capacity in vivo. Despite its importance, the FA is rarely determined in T-cell vaccination studies. We addressed the question whether different time intervals for short-term homologous vaccinations impact the FA of CD8 T-cell responses. Four-week instead of 2-week intervals between priming and boosting with potent subunit vaccines in C57BL/6 mice did not improve FA. Equally, similar FA was observed after vaccination with virus-like particles displaying low versus high antigen densities. Interestingly, FA was stable in vivo but not in vitro, depending on the antigen dose and the time interval since T-cell activation, as observed in murine monoclonal T cells. Our findings suggest dynamic in vivo modulation for equal FA. We conclude that low antigen density vaccines or a minimal 4-week prime/boost interval are not crucial for the T-cell's FA, in contrast to antibody responses. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://boris.unibe.ch/137014/ |
| Availability: |
https://boris.unibe.ch/137014/1/Gilfillan_et_al-2019-European_Journal_of_Immunology.pdf; https://boris.unibe.ch/137014/ |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.29D7258D |
| Database: |
BASE |