| Title: |
Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation in Drosophila Sensory Organ Precursor Cells |
| Authors: |
Bellec, Karen; Pinot, Mathieu; Gicquel, Isabelle; Le Borgne, Roland |
| Contributors: |
Institut de Génétique et Développement de Rennes (IGDR); Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS)-Structure Fédérative de Recherche en Biologie et Santé de Rennes (Biosit : Biologie - Santé - Innovation Technologique); ANR-16-CE13-0004 Cytosign, Agence Nationale pour La Recherche; Fondation pour la Recherche Médiclae (FRM) FDT; ANR-16-CE13-0004,CYTOSIGN,Polarité épithéliale et signalisation au cours de la cytodiérèse des cellules animales(2016) |
| Source: |
ISSN: 0950-1991. |
| Publisher Information: |
HAL CCSD; Company of Biologists |
| Publication Year: |
2021 |
| Collection: |
Université de Rennes 1: Publications scientifiques (HAL) |
| Subject Terms: |
AP-1; Asymmetric cell division; Intracellular trafficking; Notch signaling; Stratum; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Drosophila sensory organ precursors divide asymmetrically to generate pIIa/pIIb cells whose identity relies on activation of Notch at cytokinesis. While Notch is present apically and basally relative to the midbody at the pIIa-pIIb interface, the basal pool of Notch is reported to be the main contributor for Notch activation in the pIIa cell. Intra-lineage signaling requires appropriate apico-basal targeting of Notch, its ligand Delta and its trafficking partner Sanpodo. We previously reported that AP-1 and Stratum regulate the trafficking of Notch and Sanpodo from the trans-Golgi network to the basolateral membrane. Loss of AP-1 or Stratum caused mild Notch gain-of-function phenotypes. Here, we report that their concomitant loss results in a penetrant Notch gain-of-function phenotype indicating that they control parallel pathways. While unequal partitioning of cell fate determinants and cell polarity were unaffected, we observed increased amounts of signaling-competent Notch as well as Delta and Sanpodo at the apical pIIa-pIIb interface at the expense of the basal pool of Notch. We propose that AP-1 and Stratum operate in parallel pathways to localize Notch and control where receptor activation takes place. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/33298463; PUBMED: 33298463 |
| DOI: |
10.1242/dev.191437 |
| Availability: |
https://hal.science/hal-03099273; https://hal.science/hal-03099273v1/document; https://hal.science/hal-03099273v1/file/dev.191437.full.pdf; https://doi.org/10.1242/dev.191437 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.29E34A4D |
| Database: |
BASE |