| Title: |
Competitive advantage of a dengue 4 virus when co-infecting the mosquito Aedes aegypti with a dengue 1 virus. |
| Authors: |
Vazeille, Marie; Gaborit, Pascal; Mousson, Laurence; Girod, Romain; Failloux, Anna-Bella |
| Contributors: |
Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors; Institut Pasteur Paris (IP); Institut Pasteur de la Guyane; Pasteur Network (Réseau International des Instituts Pasteur); PG’s stay in Paris was supported by the Regional Health Agency in French Guiana "Convention n°09-21/GRSP du 10 août 2009". This study was funded by the Institut Pasteur and the French Government's Investissement d'Avenir program, Laboratoire d'Excellence "Integrative Biology of Emerging Infectious Diseases" (grant n°ANR-10-LABX-62-IBEID).; ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010) |
| Source: |
ISSN: 1471-2334 ; BMC Infectious Diseases ; https://pasteur.hal.science/pasteur-01473826 ; BMC Infectious Diseases, 2016, 16 (1), pp.318. ⟨10.1186/s12879-016-1666-0⟩. |
| Publisher Information: |
CCSD; BioMed Central |
| Publication Year: |
2016 |
| Collection: |
Réseau International des Instituts Pasteur, Paris: HAL-RIIP |
| Subject Terms: |
Dengue; Competitive interactions; Co-infection; Aedes aegypti; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Description: |
International audience ; BackgroundDengue viruses (DENV) are comprised in four related serotypes (DENV-1 to 4) and are critically important arboviral pathogens affecting human populations in the tropics. South American countries have seen the reemergence of DENV since the 1970’s associated with the progressive re-infestation by the mosquito vector, Aedes aegypti. In French Guiana, DENV is now endemic with the co-circulation of different serotypes resulting in viral epidemics. Between 2009 and 2010, a predominant serotype change occurred from DENV-1 to DENV-4 suggesting a competitive displacement. The aim of the present study was to evaluate the potential role of the mosquito in the selection of the new epidemic serotype.MethodsTo test this hypothesis of competitive displacement of one serotype by another in the mosquito vector, we performed mono- and co-infections of local Ae. aegypti collected during the inter-epidemic period with both viral autochthonous epidemic serotypes and compared infection, dissemination and transmission rates. We performed oral artificial infections of F1 populations in BSL-3 conditions and analyzed infection, dissemination and transmission rates.ResultsWhen two populations of Ae. aegypti from French Guiana were infected with either serotype, no significant differences in dissemination and transmission were observed between DENV-1 and DENV-4. However, in co-infection experiments, a strong competitive advantage for DENV-4 was seen at the midgut level leading to a much higher dissemination of this serotype. Furthermore only DENV-4 was present in Ae. aegypti saliva and therefore able to be transmitted.ConclusionsIn an endemic context, mosquito vectors may be infected by several DENV serotypes. Our results suggest a possible competition between serotypes at the midgut level in co-infected mosquitoes leading to a drastically different transmission potential and, in this case, favoring the competitive displacement of DENV-1 by DENV-4. This phenomenon was observed despite a similar replicative ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/27390932; PUBMED: 27390932 |
| DOI: |
10.1186/s12879-016-1666-0 |
| Availability: |
https://pasteur.hal.science/pasteur-01473826; https://pasteur.hal.science/pasteur-01473826v1/document; https://pasteur.hal.science/pasteur-01473826v1/file/art%253A10.1186%252Fs12879-016-1666-0.pdf; https://doi.org/10.1186/s12879-016-1666-0 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.2AD9F519 |
| Database: |
BASE |