| Title: |
Functional analysis of the Mps1 MAP kinase pathway in the rice blast fungus Magnaporthe grisea |
| Authors: |
Ant, C.; Chaintreuil, C.; Bonnet, C.; Lappartien, A.; Beffa, R.; Cartwright, C.; Talbot, N.; Lebrun, Marc-Henri, M.-H.; Biologie Gestion Des Risques En Agriculture - Champignons Pathogènes Des Plantes, .; Bayer Cropscience, .; Bayer Cropscience France, .; University of Exeter, . |
| Contributors: |
Centre National de la Recherche Scientifique (CNRS); Bayer S.A.S. France; Bayer AG Germany; University of Exeter; BIOlogie et GEstion des Risques en agriculture (BIOGER); Institut National de la Recherche Agronomique (INRA)-AgroParisTech |
| Source: |
8. Rencontres de Phytopathologie - Mycologie de la Société Française de Phytopathologie (SFP); https://hal.inrae.fr/hal-02817238; 8. Rencontres de Phytopathologie - Mycologie de la Société Française de Phytopathologie (SFP), Jan 2010, Aussois, France. pp.109 |
| Publisher Information: |
CCSD |
| Publication Year: |
2010 |
| Collection: |
Institut National de la Recherche Agronomique: ProdINRA |
| Subject Terms: |
development; stress survival; analyse; cascade de signalisation MAP kinase; Magnaporthe grisea; fonction; [SDV]Life Sciences [q-bio] |
| Subject Geographic: |
Aussois; France |
| Description: |
Session 4: Interactions Moleculaires ; National audience ; Cell wall integrity is crucial for fungal growth, development and stress survival. In yeast, Slt2MAP kinase and calcineurin signaling pathways monitor cell wall repair during stress and development. MPS1, the M. grisea SLT2 orthologue, is essential for cell wall repair and for appressorium mediated penetration into host plants (Xu 1998 PNAS 95:12713). In yeast, Slt2 activates the transcription factors Rlm1, Swi4 and Swi6, while calcineurin activates Crz1. Genes orthologous to yeast CRZ1, MPS1, RLM1, SWI4, and SWI6 genes were identified in M. grisea genome. Swi4 and Swi6 interact with Mps1 in yeast two hybrid assays. Deletion mutants were constructed by targeted gene replacement in M. grisea. Delta-mps1 mutants displayed an abnormal mycelial growth (no aerial hyphae), did not sporulate, and were nonpathogenic on plants as reported. Delta-crz1, delta-rlm1, delta-swi6 mutants have a normal mycelial growth and sporulation rates similar to wild type. Of these three mutants, only delta- Rlm1 displays a highly reduced pathogenicity on barley and rice (-98%, lesion number). Delta-mps1 mutants are highly sensitive to nikkomycin Z (chitin synthase inhibitor), CFW (disorganization of cell wall) and aculeacine (glucan synthase inhibitor), while delta-crz1 and delta-rlm1 mutant are only mildly hypersensitive to Nikkomycin, and delta-swi6 mutant is only slightly hypersensitive to CFW. These studies suggest that the transcription factors controlled by Mps1 are either functionally redundant or specialized in the control of specific target genes. |
| Document Type: |
conference object |
| Language: |
English |
| Relation: |
PRODINRA: 40293 |
| Availability: |
https://hal.inrae.fr/hal-02817238; https://hal.inrae.fr/hal-02817238v1/document; https://hal.inrae.fr/hal-02817238v1/file/40293_20111214090653521_1.pdf |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.2B0BC2AE |
| Database: |
BASE |