| Title: |
Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease |
| Authors: |
Reid, DT; McDonald, B; Khalid, T; Vo, T; Schenck, LP; Surette, MG; Beck, PL; Reimer, RA; Probert, CS; Rioux, KP; Eksteen, B |
| Publisher Information: |
Elsevier BV |
| Publication Year: |
2016 |
| Collection: |
The University of Liverpool Repository |
| Description: |
Background and aims: Non-alcoholic fatty liver disease is now the leading liver disease in North America. The progression of non-alcoholic fatty liver disease to the inflammatory condition, non-alcoholic steatohepatitis is complex and currently not well understood. Intestinal microbial dysbiosis has been implicated in the development of non-alcoholic fatty liver disease and progression of non-alcoholic steatohepatitis. Volatile organic compounds are byproducts of microbial metabolism in the gut that may enter portal circulation and have hepatotoxic effects contributing to the pathogenesis of non-alcoholic steatohepatitis. To test this hypothesis, we measured volatile organic compounds in cecal luminal contents and portal venous blood in a mouse model of non-alcoholic steatohepatitis. Methods: Gas chromatography-mass spectrometry analysis was conducted on cecal content and portal vein blood for volatile organic compound detection from mice fed a methionine and choline deficient diet, which induces non-alcoholic steatohepatitis. The colonic microbiome was studied by 16S rRNA gene amplification using the Illumina MiSeq platform. Results: Sixty-eight volatile organic compounds were detected in cecal luminal content, a subset of which was also present in portal venous blood. Importantly, differences in portal venous volatile organic compounds were associated with diet-induced steatohepatitis establishing a biochemical link between gut microbiota-derived volatile organic compounds and increased susceptibility to non-alcoholic steatohepatitis. Conclusion: Our model creates a novel tool to further study the role of gut-derived volatile organic compounds in the pathogenesis of non-alcoholic steatohepatitis. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
0925-4439 |
| Relation: |
https://livrepository.liverpool.ac.uk/3001580/1/Reid%20et%20al_MBD%202016_all_ChrisP.doc; Collapse authors list. Reid, DT, McDonald, B, Khalid, T, Vo, T, Schenck, LP, Surette, MG, Beck, PL, Reimer, RA, Probert, CS orcid:0000-0003-0477-6714 , Rioux, KP et al (show 1 more authors) and Eksteen, B (2016) Unique microbial-derived volatile organic compounds in portal venous circulation in murine non-alcoholic fatty liver disease Biochimica Et Biophysica Acta Molecular Basis of Disease, 1862 (7). pp. 1337-1344. ISSN 0925-4439, 1879-260X |
| DOI: |
10.1016/j.bbadis.2016.04.005 |
| Availability: |
https://livrepository.liverpool.ac.uk/3001580/; https://doi.org/10.1016/j.bbadis.2016.04.005; https://livrepository.liverpool.ac.uk/3001580/1/Reid%20et%20al_MBD%202016_all_ChrisP.doc |
| Accession Number: |
edsbas.2B169FFD |
| Database: |
BASE |