| Title: |
Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise |
| Authors: |
Smiles, WJ; Conceicao, MS; Telles, GD; Chacon-Mikahil, MPT; Cavaglieri, CR; Vechin, FC; Libardi, CA; Hawley, JA; Camera, DM |
| Publisher Information: |
Springer |
| Publication Year: |
2017 |
| Collection: |
Liverpool John Moores University: LJMU Research Online |
| Subject Terms: |
RC1200 Sports Medicine |
| Description: |
Purpose Autophagy is an intracellular degradative system sensitive to hypoxia and exercise-induced perturbations to cellular bioenergetics. We determined the effects of low-intensity endurance-based exercise performed with blood-flow restriction (BFR) on cell signaling adaptive responses regulating autophagy and substrate metabolism in human skeletal muscle. Methods In a randomized cross-over design, nine young, healthy but physically inactive males completed three experimental trials separated by 1 week of recovery consisting of either a resistance exercise bout (REX: 4 × 10 leg press repetitions, 70% 1-RM), endurance exercise (END: 30 min cycling, 70% VO2peak), or low-intensity cycling with BFR (15 min, 40% VO2peak). A resting muscle biopsy was obtained from the vastus lateralis 2 weeks prior to the first exercise trial and 3 h after each exercise bout. Results END increased ULK1Ser757 phosphorylation above rest and BFR (~37 to 51%, P < 0.05). Following REX, there were significant elevations compared to rest (~348%) and BFR (~973%) for p38γ MAPKThr180/Tyr182 phosphorylation (P < 0.05). Parkin content was lower following BFR cycling compared to REX (~20%, P < 0.05). There were no exercise-induced changes in select markers of autophagy following BFR. Genes implicated in substrate metabolism (HK2 and PDK4) were increased above rest (~143 to 338%) and BFR cycling (~212 to 517%) with END (P < 0.001). Conclusion A single bout of low-intensity cycling with BFR is insufficient to induce intracellular “stress” responses (e.g., high rates of substrate turnover and local hypoxia) necessary to activate skeletal muscle autophagy signaling. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| ISSN: |
1439-6319 |
| Relation: |
https://researchonline.ljmu.ac.uk/id/eprint/7417/1/Acute%20low-intensity%20cycling%20with%20blood-flow%20restriction%20has%20no%20effect%20on%20metabolic%20signaling%20in%20human%20skeletal%20muscle%20compared%20to%20traditional%20exerc.pdf; Smiles, WJ, Conceicao, MS, Telles, GD, Chacon-Mikahil, MPT, Cavaglieri, CR, Vechin, FC, Libardi, CA, Hawley, JA and Camera, DM (2017) Acute low-intensity cycling with blood-flow restriction has no effect on metabolic signaling in human skeletal muscle compared to traditional exercise. European Journal of Applied Physiology, 117 (2). pp. 345-358. ISSN 1439-6319 |
| DOI: |
10.1007/s00421-016-3530-8 |
| Availability: |
https://researchonline.ljmu.ac.uk/id/eprint/7417/; https://doi.org/10.1007/s00421-016-3530-8 |
| Accession Number: |
edsbas.2C37E69C |
| Database: |
BASE |