| Title: |
Reduction of cortical pulling at mitotic entry facilitates aster centration |
| Authors: |
Rosfelter, Anne; de Labbey, Ghislain; Chenevert, Janet; Dumollard, Rémi; Schaub, Sébastien; Machaty, Zoltan; Besnardeau, Lydia; Gonzalez Suarez, Daniel; Hebras, Céline; Turlier, Hervé; Burgess, David; Mcdougall, Alex |
| Contributors: |
LBDV_ASCIDIAN_BIOCELL_GROUP (abiocell); Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV); Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV); Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS); Centre interdisciplinaire de recherche en biologie (CIRB); Labex MemoLife; École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Boston College (BC) |
| Source: |
ISSN: 0021-9533. |
| Publisher Information: |
CCSD; Company of Biologists |
| Publication Year: |
2023 |
| Subject Terms: |
Sperm aster Mitotic apparatus Nuclear migration Cell cycle; Sperm aster; Mitotic apparatus; Nuclear migration; Cell cycle; [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] |
| Description: |
International audience ; Although it has been studied for more than a century, the question of how one cell divides into two equal parts is still not fully resolved. Zygotes have provided much of the mechanistic insight into how the mitotic apparatus finds the center of the cell since the centrally-located mitotic apparatus is created from a large sperm aster that forms at the cortex and thus far from the zygote center. Here we show that in ascidians, the sperm aster extends throughout the cytoplasm during interphase yet remains located near the cortex and does not migrate towards the zygote center. It is only at mitotic entry, when the sperm aster has duplicated and the mitotic apparatus is being assembled, that most of the migration and centration occurs. This temporal pattern of centration behavior is mirrored by primate zygotes (including human). The current mechanisms of aster centration include cytoplasmic pulling that scale with microtubule (MT) length, MT pushing against the proximal cortex or MT-based cortical pulling. However, it is not yet known whether and how these 3 mechanisms are coordinated to prevent aster migration during interphase and trigger migration at mitotic entry. By monitoring quantitatively all three mechanisms (cytoplasmic pulling, pushing and cortical pulling) we have discovered that cortical pulling is switched off as the zygote enters mitosis while both cytoplasmic pulling and proximal cortical pushing remain active. Physical simulations could recapitulate both the static and migratory aspects of sperm aster and mitotic apparatus behavior. We therefore surmise that the reduction in cortical pulling at mitotic entry represents a switch that allows proximal cortical pushing forces and cytoplasmic pulling forces to center the nascent mitotic apparatus. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
BIORXIV: 2023.03.21.533625 |
| DOI: |
10.1101/2023.03.21.533625 |
| Availability: |
https://cnrs.hal.science/hal-04042440; https://cnrs.hal.science/hal-04042440v1/document; https://cnrs.hal.science/hal-04042440v1/file/2023.03.21.533625v1.full.pdf; https://doi.org/10.1101/2023.03.21.533625 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.2D1AB32C |
| Database: |
BASE |