| Title: |
Lipoxin generation by human magakaryocyte-induced 12-lipoxygenase |
| Authors: |
Sheppard KA; Greenberg SM; Funk CD; Serhan CN; ROMANO, Mario |
| Contributors: |
Sheppard, Ka; Greenberg, Sm; Funk, Cd; Romano, Mario; Serhan, Cn |
| Publication Year: |
1992 |
| Collection: |
ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| Description: |
Eicosanoid biosynthesis was examined with a human megakaryocytic cell line (Dami). Megakaryocytes incubated with [1-14C]arachidonic acid and either ionophore A23187 or thrombin generated both thromboxane and 12-hydroxyheptadecatrienoic acid (HHTrE). Exposure to phorbol myristate acetate (PMA) for 1 through 9 days induced differentiation and revealed an increase in the conversion of [1-14C]arachidonate to cyclooxygenase- and lipoxygenase (LO)-derived products. The LO-derived product was identified as 12S-HETE by its physical characteristics including GC/MS and chiral column SP-HPLC. PMA-treated Dami cells did not generate 5-HETE, leukotrienes or lipoxins from exogenous arachidonic acid while they did convert leukotriene A4 (LTA4) to lipoxin A4, lipoxin B4 and their respective all-trans isomers. In addition, COS-M6 cells transfected with a human 12-lipoxygenase cDNA and incubated with either arachidonic acid or LTA4 generated 12-HETE and lipoxins, respectively. The lipoxin profile generated by transfected COS-M6 cells incubated with LTA4 was similar to that generated by the PMA-treated Dami cells. Results indicate that human megakaryocytes can transform arachidonate and LTA4 to bioactive eicosanoids and that the 12-lipoxygenase appears upon further differentiation of these cells. In addition, they indicate that the 12-LO of human megakaryocytes and the 12-LO expressed by transfected COS cells can generate both lipoxins A4 and B4. Together they suggest that the human 12-LO can serve as a model of LX-synthetase activity with LTA4. |
| Document Type: |
article in journal/newspaper |
| File Description: |
STAMPA |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/1310055; info:eu-repo/semantics/altIdentifier/wos/A1992HC28300017; volume:1133; issue:2; firstpage:223; lastpage:234; numberofpages:12; journal:BIOCHIMICA ET BIOPHYSICA ACTA; https://hdl.handle.net/11564/108187; http://www.sciencedirect.com/science/article/pii/016748899290073K |
| DOI: |
10.1016/0167-4889(92)90073-K |
| Availability: |
https://hdl.handle.net/11564/108187; https://doi.org/10.1016/0167-4889(92)90073-K; http://www.sciencedirect.com/science/article/pii/016748899290073K |
| Accession Number: |
edsbas.2D3EA89 |
| Database: |
BASE |