| Title: |
P-291. Whole genome sequencing (WGS) demonstrates high relatedness between carbapenemase-producing Klebsiella pneumoniae isolates cultured from paired rectal swab and blood specimens among hospitalized patients in Greece |
| Authors: |
Banerjee, Ritu; Greenwood-Quaintance, Kerryl; Koscianski, Christina; Starkey, Jordan; Rodning, Amanda; Patel, Robin; Mantzana, Paraskevi; Tychala, Areti; Meletis, Georgios; Sereni, Zoe; Trikoupis, Petros; Oikonomou, Margarita; Kousouli, Elisavet; Roussou, Anna; Charami, Smaragdi; Rovatsou, Alexandra; Louka, Christina; Mamali, Vasiliki; Chrysos, Georgios; Alexandros Baziotis, Georgios; Vourli, Sophia; Christina Georgiou, Panagiota; Louka, Styliani; Poulianou, Eleni; Karakosta, Polyxeni; Protonotariou, Efthymia; Pournaras, Spyros; Zarkotou, Olympia; Skoura, Lemonia |
| Source: |
Open Forum Infectious Diseases ; volume 12, issue Supplement_1 ; ISSN 2328-8957 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2025 |
| Description: |
Background Intestinal colonization with carbapenem-resistant organisms (CRO) may be considered to inform empiric antibiotic selection for symptomatic infection. However, it is unclear how often gut colonizing CRE isolates are related to bloodstream isolates in hospitalized patients with bacteremia, especially in areas with high CRO prevalence. Minimum spanning tree of isolate relatedness Isolates and corresponding patient number (P1-P6) and rectal (R) or blood (B) specimen are noted within circles and allelic differences are numerated on linking lines between the circles. Group 1 and 3 isolates are possibly related to each other. Created with BioRender.com. Methods Patients with paired K. pneumoniae carbapenemase (KPC)-producing K. pneumoniae isolates cultured from surveillance rectal specimens and clinically collected blood specimens collected within 1-8 days of each other between 2019-2023 were identified at 3 Greek hospitals. Isolates were frozen and shipped to a reference laboratory in the U.S for whole genome sequencing (WGS) using Illumina MiSeq. Assembly and core genome multilocus sequence typing (cgMLST) analysis were performed with Ridom SeqSphere+ software. Isolate relatedness was based on the total number of allelic differences between each isolate: < 15, related; 16-50, possibly related; ≥ 51, unrelated. Results One hundred eight paired isolates were identified from 54 unique patients; 6 pairs and 12 total isolates underwent cgMLST. In 5 of 6 pairs (83%), the rectal isolate was collected prior to the bloodstream isolate. Rectal and bloodstream isolates were related to one another in 5 of 6 (83%) pairs and were unrelated in 1 pair. Two pairs collected from 2 distinct patients admitted to the same intensive care unit (patients 2 and 3, Figure) were possibly related (16-50 allelic differences) to each other. Conclusion In this pilot evaluation conducted in a setting of high CRO prevalence, KPC-producing K. pneumoniae isolated from paired rectal and blood specimens from individual ... |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/ofid/ofae631.494 |
| Availability: |
https://doi.org/10.1093/ofid/ofae631.494; https://academic.oup.com/ofid/article-pdf/12/Supplement_1/ofae631.494/61680685/ofae631.494.pdf |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.2D4C7650 |
| Database: |
BASE |