| Title: |
Molecular and genetic determinism of sorghum grain quality |
| Authors: |
Sene, Mamadou; Calatayud, Caroline; Berger, Angélique; Soriano, Alexandre; Richaud, Frédérique; de Bellis, Fabien; Sotillo, Armel; Rios, Maelle; Bonicel, Joëlle; Frouin, Julien; Singer, Mathilde; Bonnal, Laurent; Mameri, Hamza; Pot, David; Terrier, Nancy |
| Contributors: |
Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP); Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM); Université de Montpellier (UM); Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Systèmes d'élevage méditerranéens et tropicaux (UMR SELMET); Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Compte d'affectation spécial Développement agricole et rural; CASDAR; FRA; Ministère français de l'Agriculture et de la l'Alimentation |
| Source: |
CBB8 2025 8th Conference on Cereal Biotechnology and Breeding, EWAC19 2025 The European Cereals Genetics Co-opeative Conference - Book of abstracts ; Conference on Cereal Biotechnology and Breeding ; https://hal.science/hal-05394372 ; Conference on Cereal Biotechnology and Breeding, Nov 2025, Budapest, Hungary. pp.69-69 |
| Publisher Information: |
CCSD; AKCongress |
| Publication Year: |
2025 |
| Subject Terms: |
GWAS; Transcription factor; Protoplast; Gene co-expression network; Protein digestibility; Protein; Starch; Grain quality; Sorghum; [SDV]Life Sciences [q-bio] |
| Subject Geographic: |
Budapest; Hungary |
| Description: |
Source Agritrop Cirad (https://agritrop.cirad.fr/615882/) * Autres projets (id;sigle;titre): ;NitroSorg;(FRA) Développer une boite à outil pour la sélection de variétés de sorgho maximisant leur qualité de grains (teneur et digestibilité des protéines) et leur adaptation à l’alimentation des volailles// ; International audience ; Sorghum grains are rich in proteins and starch but exhibit low protein digestibility, limiting their use in food and feed. However, the genetic and molecular mechanisms underlying these traits remain poorly understood, particularly the genomic regions involved, as well as the structural genes and transcription factors (TFs) that regulate them, hindering efforts to improve sorghum grain quality. To address this, we adopted an integrated genomic and genetic approach. At the genomic level, we constructed a gene co-expression network using transcriptomic data collected during grain development (10 stages) over two years. In parallel, we quantified starch and protein content and measured protein digestibility. Two major gene co-expression modules were identified. The first was linked to the loss of protein digestibility, involving genes related to disulfide bond formation and modulation. The second contained most kafirin and starch metabolism genes, as well as orthologs of TFs known to regulate protein and starch accumulation in other cereal species. Functional assays performed in protoplasts for six TFs revealed a central role for SbPBF1a, SbPBF1b, and SbNF-YC13 in modulating the expression of genes involved in protein and starch biosynthesis. In the genetic approach, we used a diversity panel of 300 sorghum genotypes representing global genetic diversity. This panel was cultivated at two sites in France over two growing seasons. Protein, starch contents and protein digestibility were measured. Whole-genome sequencing (WGS) was performed for all genotypes. These biochemical and genotypic data were analysed through genome-wide association studies (GWAS) using both single and multilocus ... |
| Document Type: |
conference object |
| Language: |
English |
| Relation: |
CIRAD: 615882 |
| Availability: |
https://hal.science/hal-05394372; https://hal.science/hal-05394372v1/document; https://hal.science/hal-05394372v1/file/615882_abstract.pdf |
| Rights: |
http://hal.archives-ouvertes.fr/licences/copyright/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.2D87F961 |
| Database: |
BASE |