| Title: |
Cell wall arabinogalactan is responsible for Fungitell® cross reactivity in nocardiosis. |
| Authors: |
Mariller, Christophe; Letowski, Pascal; Chang, W. T.; Lowary, T. L.; Ulrich, Marc; Faure, Karine; Loridant, Severine; Sendid, Boualem; Wallet, Frederic; Poulain, Daniel; Hazzan, Marc; Frimat, Marie; Guerardel, Yann; Titecat, Marie |
| Contributors: |
Université de Lille; Inserm; CHU Lille; Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 UGSF; Centre hospitalier Valenciennes, Nord; Centre Hospitalier Régional Universitaire CHU Lille CHRU Lille; Institute for Translational Research in Inflammation - U 1286 INFINITE; Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 RID-AGE |
| Publisher Information: |
Elsevier |
| Publication Year: |
2025 |
| Collection: |
LillOA (Lille Open Archive - Université de Lille) |
| Description: |
Nocardiosis is a serious infection in immunosuppressed patients, especially transplant recipients. The slow-growing phenotype of the bacterium and the variety of symptoms complicate diagnosis and delay antimicrobial therapy, resulting in high mortality rates despite effective treatments. A further complication is that some nocardiosis patients test positive in fungal diagnostics that detect (1,3)-β-D-glucan (the Fungitell® assay), but the basis for this cross-reactivity remains unknown. We demonstrate that nocardial cell wall arabinogalactan is a cryptic antigen responsible for cross-reactivity in the Fungitell® assay and that this antigen is revealed in vivo following bacterial cell lysis. We further show that the reactivity results from a β-glucose substitution of the galactan domain, a modification specific to nocardia, and identify the optimal antigen as a tetramer of the trisaccharide repeating unit. By providing structural evidence for Fungitell® cross-reactivity during nocardiosis, this work paves the way for developing specific diagnostic tools that are currently lacking. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/octet-stream |
| Language: |
English |
| Relation: |
Journal of Biological Inorganic Chemistry; J Biol Chem |
| Availability: |
https://lilloa.univ-lille.fr/handle/20.500.12210/131758; https://hdl.handle.net/20.500.12210/131758 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.2DAB5D20 |
| Database: |
BASE |