| Title: |
Single-cell signaling network profiling during redox stress reveals dynamic redox regulation in immune cells |
| Authors: |
Yi-Chuan Wang; Ping-Hsun Wu; Wen-Chieh Ting; Yi-Fu Wang; Ming-Han Yang; Tung-Hung Su; Jia-Ying Su; Hsun-I Sun; Wei-Min Huang; Pei-Ling Tsai; Gerlinde Wernig; Ping-Chih Ho; Limei Wang; Chen-Tu Wu; Yih-Leong Chang; Tseng-Cheng Chen; Tzu-Ching Meng; Yao-Ming Chang; Shih-Lei Lai; Chia-Wei Li; Tai-Ming Ko; Kai-Chien Yang; Ya-Jen Chang; Yijuang Chern; Mei-Chuan Kuo; Yen-Tsung Huang; Yi-Shiuan Tzeng; Jih-Luh Tang; Shih-Yu Chen |
| Source: |
Nature Communications, Vol 16, Iss 1, Pp 1-18 (2025) |
| Publisher Information: |
Nature Portfolio |
| Publication Year: |
2025 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Science |
| Description: |
In eukaryotic cells, reactive oxygen species (ROS) serve as crucial signaling components. ROS are potentially toxic, so constant adjustments are needed to maintain cellular health. Here we describe a single-cell, mass cytometry-based method that we call signaling network under redox stress profiling (SN-ROP) to monitor dynamic changes in redox-related pathways during redox stress. SN-ROP quantifies ROS transporters, enzymes, oxidative stress products and associated signaling pathways to provide information on cellular redox regulation. Applied to diverse cell types and conditions, SN-ROP reveals unique redox patterns and dynamics including coordinated shifts in CD8+ T cells upon antigen stimulation as well as variations in CAR-T cell persistence. Furthermore, SN-ROP analysis uncovers environmental factors such as hypoxia and T cell exhaustion for influencing redox balance, and also reveals distinct features in patients on hemodialysis. Our findings thus support the use of SN-ROP to elucidate intricate redox networks and their implications in immune cell function and disease. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.1038/s41467-025-60727-z; https://doaj.org/toc/2041-1723; https://doaj.org/article/d3606999b8d1404fb58ca23cabc5bb7e |
| DOI: |
10.1038/s41467-025-60727-z |
| Availability: |
https://doi.org/10.1038/s41467-025-60727-z; https://doaj.org/article/d3606999b8d1404fb58ca23cabc5bb7e |
| Accession Number: |
edsbas.2E07CE99 |
| Database: |
BASE |