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Oxytocin‐loaded hydrogel promotes cartilage regeneration and regulates microenvironment

Title: Oxytocin‐loaded hydrogel promotes cartilage regeneration and regulates microenvironment
Authors: Wang, Tianming; Zhao, Xiao; Li, Jiayi; Yin, Chongchong; Jiang, Bo; Xie, Jiaming; Wang, Binghao; Wang, Yufeng; Cao, Zhicheng; Yao, Qingqiang; Zheng, Shengnai; Sui, Jisheng; Zhu, Kun
Contributors: Science and Technology Project of Jiangsu Province; Health research project of Anhui Province; National Natural Science Foundation of China; Excellent scientific research and innovation team of Anhui universities; Nanjing Science and Technology Plan Project; Natural Science Research Project of Anhui Educational Committee; Medical Innovation Foundation from Spinal deformity clinical and research center of Anhui province; Nature Science Foundation of Jiangsu Province; Nanjing International Joint Research and Development Project; Research and Development Program of China
Source: Biofabrication ; volume 17, issue 2, page 025030 ; ISSN 1758-5082 1758-5090
Publisher Information: IOP Publishing
Publication Year: 2025
Description: Osteoarthritis is a common orthopedic condition, and traditional treatment methods often fail to regenerate cartilage effectively. Oxytocin (OXT) is a neuropeptide that plays a crucial role in the skeletal system. Hyaluronic acid (HAMA) hydrogel has emerged as a key carrier for cartilage repair due to its excellent biocompatibility and biodegradability. Combining OXT with HAMA hydrogel and implanting it at the site of cartilage defects can effectively promote cartilage regeneration. Cartilage damage often results in an altered microenvironment, characterized by macrophage polarization and high levels of reactive oxygen species (ROS). Oxidative stress can stimulate macrophages to produce more pro-inflammatory factors. OXT can inhibit the secretion of pro-inflammatory cytokines such as TNF- α , IL-6, and IL-1 β by interacting with the STAT3/NF- κ B signaling pathway, as well as the PI3K/Akt and mitogen-activated protein kinase pathways, thereby inducing the polarization of macrophages from the M1 phenotype to the M2 phenotype and alleviating the inflammatory response. OXT can also enhance the expression of NRF and HO-1, which helps eliminate ROS and suppress the expression of pro-inflammatory factors. Regulating the microenvironment of cartilage damage is beneficial for cartilage protection and repair. OXT activates the CFOS/AP-1 and STAT1/JAK2 pathways, which together act on MMP2 and MMP9 to alleviate cartilage degeneration. The STAT1/JAK2 pathway can further increase the expression of Col2, thereby protecting chondrocytes. Additionally, OXT can directly boost the protein levels of SOX9 and COMP, promoting chondrocyte proliferation and cartilage protection, ultimately achieving the therapeutic goal for arthritis. This study explores the potential of HAMA hydrogel as a delivery system for OXT and analyzes their impact on cartilage regeneration and anti-inflammatory properties. This research provides a novel strategy for the treatment of cartilage injuries.
Document Type: article in journal/newspaper
Language: unknown
DOI: 10.1088/1758-5090/adc158
DOI: 10.1088/1758-5090/adc158/pdf
Availability: https://doi.org/10.1088/1758-5090/adc158; https://iopscience.iop.org/article/10.1088/1758-5090/adc158; https://iopscience.iop.org/article/10.1088/1758-5090/adc158/pdf
Rights: https://creativecommons.org/licenses/by/4.0/ ; https://iopscience.iop.org/info/page/text-and-data-mining
Accession Number: edsbas.2E48D72F
Database: BASE