| Title: |
A Proteomic Approach for Systematic Mapping of Substrates of Human Deubiquitinating Enzymes |
| Authors: |
Ramírez Sánchez, Juan Manuel; Prieto Agujeta, Gorka; Olazabal Herrero, Ane; Borràs, Eva; Fernandez-Vigo, Elvira; Alduntzin Egizurain, Unai; Osinalde Moraleja, Nerea; Beaskoetxea Lejarzegi, Javier; Lectez, Benoit; Aloria Escolastico, Kerman; Rodríguez Pérez, José Antonio; Paradela, Alberto; Sabidó, Eduard; Muñoz, Javier; Corrales, Fernando; Arizmendi Bastarrika, Jesús María; Mayor Martínez, Ugo |
| Publisher Information: |
MDPI |
| Publication Year: |
2021 |
| Collection: |
ADDI: Repositorio Institucional de la Universidad del País Vasco / Euskal Herriko Unibertsitatea (UPV/EHU - Basque Country University) |
| Subject Terms: |
ubiquitination; deubiquitinating enzyme; quantitative proteomics; DUBase |
| Description: |
The human genome contains nearly 100 deubiquitinating enzymes (DUBs) responsible for removing ubiquitin moieties from a large variety of substrates. Which DUBs are responsible for targeting which substrates remain mostly unknown. Here we implement the bioUb approach to identify DUB substrates in a systematic manner, combining gene silencing and proteomics analyses. Silencing of individual DUB enzymes is used to reduce their ubiquitin deconjugating activity, leading to an increase of the ubiquitination of their substrates, which can then be isolated and identified. We report here quantitative proteomic data of the putative substrates of 5 human DUBs. Furthermore, we have built a novel interactive database of DUB substrates to provide easy access to our data and collect DUB proteome data from other groups as a reference resource in the DUB substrates research field. ; Authors of this paper are members of Proteored, PRB3 and are supported by grant PT17/0019 of the PE I+D+i 2013-2016, funded by Instituto de Salud Carlos III (ISCIII) and European Regional Development Fund (ERDF). This research was also funded by the Spanish Ministry of Economy, grant number SAF2016-76898-P and the University of the Basque Country (UPV/EHU), grant number US19/05. J.R was supported with a postdoctoral research fellowship from the UPV/EHU. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
info:eu-repo/grantAgreement/MINECO/SAF2016-76898-P; https://www.mdpi.com/1422-0067/22/9/4851/htm; International Journal of Molecular Sciences 22(9) : (2021) // Article ID 4851; https://hdl.handle.net/10810/51415 |
| DOI: |
10.3390/ijms22094851 |
| Availability: |
https://hdl.handle.net/10810/51415; https://doi.org/10.3390/ijms22094851 |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/3.0/es/ ; 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
| Accession Number: |
edsbas.2E6E6341 |
| Database: |
BASE |