| Title: |
HOX gene expression in the developing human spine. |
| Authors: |
Lawrence, John EG; Roberts, Kenny; Tuck, Elizabeth; Li, Tong; Mamanova, Lira; Balogh, Petra; Usher, Inga; Piapi, Alice; Mazin, Pavel; Anderson, Nathaniel D; Bolt, Liam; Richardson, Laura; Prigmore, Elena; He, Xiaoling; Barker, Roger A; Flanagan, Adrienne; Young, Matthew D; Teichmann, Sarah A; Bayraktar, Omer; Behjati, Sam |
| Publisher Information: |
Springer Nature; //doi.org/10.1038/s41467-024-54187-0 |
| Publication Year: |
2024 |
| Collection: |
Apollo - University of Cambridge Repository |
| Subject Terms: |
Humans; Gene Expression Regulation; Developmental; Genes; Homeobox; Spine; Homeodomain Proteins; Single-Cell Analysis; Spinal Cord; Transcriptome; Gene Expression Profiling |
| Description: |
Acknowledgements: We thank Professor Denis Duboule and Professor James Briscoe for their invaluable advice for this study. This study was supported by the Wellcome Trust through institutional / programme grants and personal Fellowships (WT211276/Z/18/Z- institutional grant, 108413/A/15/D (S.B. & O.B.), 222902/Z/21/Z (S.B.) and 223135/Z/21/Z (J.E.L.). ; Positional coding along the anterior-posterior axis is regulated by HOX genes, whose 3' to 5' expression correlates with location along this axis. The precise utilisation of HOX genes in different human cell types is not fully understood. Here, we use single-cell and spatial-transcriptomics, along with in-situ sequencing, to create a developmental atlas of the human fetal spine. We analyse HOX gene expression across cell types during development, finding that neural-crest derivatives unexpectedly retain the anatomical HOX code of their origin while also adopting the code of their destination. This trend is confirmed across multiple organs. In the axial plane of the spinal cord, we find distinct patterns in the ventral and dorsal domains, providing insights into motor pool organisation and loss of collinearity in HOXB genes. Our findings shed new light on HOX gene expression in the developing spine, highlighting a HOX gene 'source code' in neural-crest cell derivatives. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text/xml; application/pdf; application/zip |
| Language: |
English |
| Relation: |
54187; https://www.repository.cam.ac.uk/handle/1810/376540 |
| Availability: |
https://www.repository.cam.ac.uk/handle/1810/376540 |
| Rights: |
Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.2ED53D4A |
| Database: |
BASE |