| Title: |
Expression of the alpha subunit of PABA peptide hydrolase (EC 3.4.24.18) in MDCK cells ; Synthesis and secretion of an enzymatically inactive homodimer |
| Authors: |
Grünberg, Jürgen; Dumermuth, Eric; Eldering, Joyce A.; Sterchi, Erwin E. |
| Source: |
FEBS Letters ; volume 335, issue 3, page 376-379 ; ISSN 0014-5793 1873-3468 |
| Publisher Information: |
Wiley |
| Publication Year: |
1993 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
In this paper, we report the expression of PPHα in the polarized cell line MDCK (Madin Darby canine kidney). In these cells, the enzyme was synthesized m an inactive profonn, which upon treatment with trypsin was activated. The enzyme isolated from cell extracts was core‐glycosylated and appeared to be retained in the ER as a homodimer. No PPHα was detectable on the surface of intact cells by immunofluoreseence. However, a complex glycosylated soluble but inactive form was present in the culture medium, suggesting that proteolytic removal of the C‐terminal membrane anchoring peptide leads to the secretion of PPHα. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1016/0014-5793(93)80422-q |
| DOI: |
10.1016/0014-5793%2893%2980422-Q |
| Availability: |
https://doi.org/10.1016/0014-5793(93)80422-q; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793%2893%2980422-Q; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1016%2F0014-5793(93)80422-Q; https://febs.onlinelibrary.wiley.com/doi/pdf/10.1016/0014-5793%2893%2980422-Q |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.2FF810DF |
| Database: |
BASE |