| Title: |
Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy |
| Authors: |
Grigorov, Artem; Bychenko, Oksana; Salina, Elena G.; Skvortsova, Yulia; Mazurova, Arina; Skvortsov, Timofey; Kaprelyants, Arseny; Azhikina, Tatyana |
| Source: |
Grigorov, A, Bychenko, O, Salina, E G, Skvortsova, Y, Mazurova, A, Skvortsov, T, Kaprelyants, A & Azhikina, T 2021, 'Small RNA F6 Provides Mycobacterium smegmatis Entry into Dormancy', International Journal of Molecular Sciences, vol. 22, no. 21, 11536. https://doi.org/10.3390/ijms222111536 |
| Publication Year: |
2021 |
| Collection: |
Queen's University Belfast: Research Portal |
| Subject Terms: |
Mycobacterium smegmatis; small non-coding RNA; F6; resuscitation promoting factor RpfE2; dormancy; non-culturability; adaptation to stresses |
| Description: |
Regulatory small non-coding RNAs play a significant role in bacterial adaptation to changing environmental conditions. Various stresses such as hypoxia and nutrient starvation cause a reduction in the metabolic activity of Mycobacterium smegmatis, leading to entry into dormancy. We investigated the functional role of F6, a small RNA of M. smegmatis, and constructed an F6 deletion strain of M. smegmatis. Using the RNA-seq approach, we demonstrated that gene expression changes that accompany F6 deletion contributed to bacterial resistance against oxidative stress. We also found that F6 directly interacted with 5′-UTR of MSMEG_4640 mRNA encoding RpfE2, a resuscitation-promoting factor, which led to the downregulation of RpfE2 expression. The F6 deletion strain was characterized by the reduced ability to enter into dormancy (non-culturability) in the potassium deficiency model compared to the wild-type strain, indicating that F6 significantly contributes to bacterial adaptation to non-optimal growth conditions. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1661-6596 |
| Relation: |
info:eu-repo/semantics/altIdentifier/pissn/1661-6596 |
| DOI: |
10.3390/ijms222111536 |
| Availability: |
https://pure.qub.ac.uk/en/publications/2582c6e5-b8d4-40d0-82dd-82d99649e2c8; https://doi.org/10.3390/ijms222111536; https://pureadmin.qub.ac.uk/ws/files/258142686/ijms-22-11536-v2.pdf |
| Rights: |
info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.304AFB7 |
| Database: |
BASE |