| Title: |
Community‐Engaged Course‐Based Undergraduate Research of Multidrug Resistance in Escherichia coli in Water Near Dairy and Hog Farms in Michigan |
| Authors: |
Chowdhry, Renee; Jun, Soeun; Kong, Yuwei; Casillas Saenz, Adrian; Chung, Jonathan; Chang, Michelle; Osborn, Katie; Zhang, Yuhui; Bodeau, Will; Curristan, Nicole; Sofro, Brynn; Ray, Soham; Jimenez, Karina; Henning, Lynn; Dickerson, Cole; Jaberi, Salman; Delucchi, Clare; Miranda, Jose Reyes; Jones, Adriane; Bascom‐Slack, Carol; Jay, Jennifer A. |
| Contributors: |
Teagle Foundation; National Science Foundation |
| Source: |
Environmental Microbiology Reports ; volume 17, issue 4 ; ISSN 1758-2229 1758-2229 |
| Publisher Information: |
Wiley |
| Publication Year: |
2025 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
Antimicrobial resistance (AMR) was assessed in Michigan surface waters impacted by dairies, swine farms and human wastewater, as well as in an unimpacted (UI) comparison site. Escherichia coli (EC) was quantified in the presence and absence of cefotaxime, as extended‐spectrum beta‐lactamase‐producing EC (ESBL‐EC) has been deemed a proxy for AMR. Purified isolates of EC selected without antibiotics were characterised by disk diffusion; antibiotics tested included ampicillin, tetracycline, cefotaxime, cefoxitin, streptomycin, nalidixic acid, kanamycin, ciprofloxacin and erythromycin. Ampicillin and tetracycline resistance ranged up to 67% and 62% of the EC isolates, respectively, at livestock‐impacted sites, but were low at UI. Multidrug resistance (MDR) was not observed at all at UI but was observed in up to 76% and 67% of isolates from dairy and swine/dairy, respectively. AMP‐TE‐E was the most common resistance pattern observed, with all isolates originating from one of the dairy sites. Notably, resistance to cefotaxime did not correlate with MDR, indicating that preselection for ESBL‐EC before further AMR testing will not successfully characterise AMR or MDR from culturable EC. Interestingly, the percent of isolates resistant to AMP correlated quite well with MDR. This work highlights the importance of MDR characterisation at livestock‐impacted surface water sites. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1111/1758-2229.70151 |
| Availability: |
https://doi.org/10.1111/1758-2229.70151; https://sfamjournals.onlinelibrary.wiley.com/doi/pdf/10.1111/1758-2229.70151 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.3054F4FE |
| Database: |
BASE |