| Title: |
Proteomic as an Exploratory Approach to Develop Vaccines Against Tick-Borne Diseases Using Lyme Borreliosis as a Test Case |
| Authors: |
Talagrand-Reboul, Émilie; Westermann, Benoît; Raess, Matthieu; Schnell, Gilles; Cantero, Paola; Barthel, Cathy; Ehret-Sabatier, Laurence; Jaulhac, Benoit; Boulanger, Nathalie |
| Contributors: |
Virulence bactérienne précoce : fonctions cellulaires et contrôle de l'infection aiguë et subaiguë (VBP); Université de Strasbourg (UNISTRA); Laboratoire de Spectrométrie de Masse BioOrganique Strasbourg (LSMBO); Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC); Institut Pluridisciplinaire Hubert Curien (IPHC); Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Institut Pluridisciplinaire Hubert Curien (IPHC); Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS); Centre National de référence des Borrelia (CNR); Hôpitaux Universitaires de Strasbourg (HUS); ANR-10-SATT-0001,SATT CONECTUS,SATT CONECTUS(2010); ANR-10-INBS-0008,ProFI,Infrastructure Française de Protéomique(2010) |
| Source: |
EISSN: 2076-393X ; Vaccines ; https://hal.science/hal-02962474 ; Vaccines, 2020, 8 (3), pp.463. ⟨10.3390/vaccines8030463⟩ |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2020 |
| Collection: |
HAL-IN2P3 (Institut national de physique nucléaire et de physique des particules) |
| Subject Terms: |
tick-borne diseases; skin; proteomics; markers of infection; Lyme; Borrelia; [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases |
| Description: |
International audience ; Tick-borne diseases affecting humans and animals are on the rise worldwide. Vaccines constitute an effective control measure, but very few are available. We selected Lyme borreliosis, a bacterial infection transmitted by the hard tick Ixodes, to validate a new concept to identify vaccine candidates. This disease is the most common tick-borne disease in the Northern Hemisphere. Although attempts to develop a vaccine exist, none have been successfully marketed. In tick-borne diseases, the skin constitutes a very specific environment encountered by the pathogen during its co-inoculation with tick saliva. In a mouse model, we developed a proteomic approach to identify vaccine candidates in skin biopsies. We identified 30 bacterial proteins after syringe inoculation or tick inoculation of bacteria. Discovery proteomics using mass spectrometry might be used in various tick-borne diseases to identify pathogen proteins with early skin expression. It should help to better develop sub-unit vaccines based on a cocktail of several antigens, associated with effective adjuvant and delivery systems of antigens. In all vector-borne diseases, the skin deserves further investigation to better define its role in the elaboration of protective immunity against pathogens. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.3390/vaccines8030463 |
| Availability: |
https://hal.science/hal-02962474; https://hal.science/hal-02962474v1/document; https://hal.science/hal-02962474v1/file/Talagrand-Reboul_Vaccines%202020.pdf; https://doi.org/10.3390/vaccines8030463 |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.305F8CED |
| Database: |
BASE |