| Title: |
Dietary n-3 polyunsaturated fatty acids alter the number, fatty acid profile and coagulatory activity of circulating and platelet-derived extracellular vesicles: a randomized, controlled crossover trial |
| Authors: |
Bozbas, E; Zhou, R; Soyama, S; Allen-Redpath, K; Mitchell, JL; Fisk, HL; Calder, PC; Jones, C; Gibbins, JM; Fischer, R; Hester, S; Yaqoob, P |
| Contributors: |
https://creativecommons.org/licenses/by/4.0/ |
| Publisher Information: |
Elsevier on behalf of American Society for Nutrition |
| Publication Year: |
2024 |
| Collection: |
Brunel University London: Brunel University Research Archive (BURA) |
| Subject Terms: |
cardiovascular disease; extracellular vesicles; platelet-derived extracellular vesicles; fish oil; thrombosis; coagulation |
| Description: |
Data availability: Data described in the manuscript will be made publicly and freely available without restriction from the University of Reading Research Data Archive at [URL: https://doi.org/10.17864/1947.000513] ; Supplementary data are available online at: https://www.sciencedirect.com/science/article/pii/S0002916524003435?via%3Dihub#appsec1 . ; Background: Extracellular vesicles (EVs) are proposed to play a role in the development of cardiovascular diseases (CVDs) and are considered emerging markers of CVDs. n-3 PUFAs are abundant in oily fish and fish oil and are reported to reduce CVD risk, but there has been little research to date examining the effects of n-3 PUFAs on the generation and function of EVs. Objectives: We aimed to investigate the effects of fish oil supplementation on the number, generation, and function of EVs in subjects with moderate risk of CVDs. Methods: A total of 40 participants with moderate risk of CVDs were supplemented with capsules containing either fish oil (1.9 g/d n-3 PUFAs) or control oil (high-oleic safflower oil) for 12 wk in a randomized, double-blind, placebo-controlled crossover intervention study. The effects of fish oil supplementation on conventional CVD and thrombogenic risk markers were measured, along with the number and fatty acid composition of circulating and platelet-derived EVs (PDEVs). PDEV proteome profiles were evaluated, and their impact on coagulation was assessed using assays including fibrin clot formation, thrombin generation, fibrinolysis, and ex vivo thrombus formation. Results: n-3 PUFAs decreased the numbers of circulating EVs by 27%, doubled their n-3 PUFA content, and reduced their capacity to support thrombin generation by >20% in subjects at moderate risk of CVDs. EVs derived from n-3 PUFA-enriched platelets in vitro also resulted in lower thrombin generation, but did not alter thrombus formation in a whole blood ex vivo assay. Conclusions: Dietary n-3 PUFAs alter the number, composition, and function of EVs, reducing their coagulatory ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
1175 - 1186; Print-Electronic |
| Language: |
English |
| Relation: |
American Journal of Clinical Nutrition; https://bura.brunel.ac.uk/handle/2438/29133; https://doi.org/10.1016/j.ajcnut.2024.03.008 |
| DOI: |
10.1016/j.ajcnut.2024.03.008 |
| Availability: |
https://bura.brunel.ac.uk/handle/2438/29133; https://doi.org/10.1016/j.ajcnut.2024.03.008 |
| Rights: |
Copyright © 2024 The Author(s). Published by Elsevier Inc. on behalf of American Society for Nutrition. This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). ; https://creativecommons.org/licenses/by/4.0/legalcode.en ; The Author(s) |
| Accession Number: |
edsbas.30B378DF |
| Database: |
BASE |