| Title: |
Antagonistic Effect of Sucrose Availability and Auxin on Rosa Axillary Bud Metabolism and Signaling, Based on the Transcriptomics and Metabolomics Analysis |
| Authors: |
Wang, Ming; Ogé, Laurent; Pérez Garcia, Maria-Dolores; Launay-Avon, Alexandra; Clément, Gilles; Le Gourrierec, Jose; Hamama, Latifa; Sakr, Soulaiman |
| Contributors: |
Institut de Recherche en Horticulture et Semences (IRHS); Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro); Qingdao Agricultural University Qingdao; Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers; Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)); Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Institut Jean-Pierre Bourgin - Sciences du végétal (IJPB); AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); IRHS - Équipe QuaRVeg (Qualité et Résistance aux Bioagresseurs des Espèces Légumières) (IRHS-QUARVEG); Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université d'Angers (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Rennes Angers; IRHS - Équipe STRAGENE (Structure et Ramification en réponse à l'Interaction Génotype et Environnement) (IRHS-STRAGENE); China Scholarships Council (no. 201506320203); Well-Breed Engineering of Shandong province (2021LZGC022); Talent Introduction Special Funds of Qingdao Agricultural University (663/1120070); ANR-15-LCV3-0002,ESTIM,Évaluation de STIMulateurs de vitalité des plantes(2015) |
| Source: |
ISSN: 1664-462X ; Frontiers in Plant Science ; https://u-paris.hal.science/hal-03671503 ; Frontiers in Plant Science, 2022, 13, pp.830840. ⟨10.3389/fpls.2022.830840⟩. |
| Publisher Information: |
CCSD; Frontiers |
| Publication Year: |
2022 |
| Collection: |
Institut National de la Recherche Agronomique: ProdINRA |
| Subject Terms: |
bud outgrowth; sucrose; auxin; signaling pathway; TOR-kinase; [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics; [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Genomics [q-bio.GN] |
| Description: |
International audience ; Shoot branching is crucial for successful plant development and plant response to environmental factors. Extensive investigations have revealed the involvement of an intricate regulatory network including hormones and sugars. Recent studies have demonstrated that two major systemic regulators-auxin and sugar-antagonistically regulate plant branching. However, little is known regarding the molecular mechanisms involved in this crosstalk. We carried out two complementary untargeted approaches-RNA-seq and metabolomics-on explant stem buds fed with different concentrations of auxin and sucrose resulting in dormant and non-dormant buds. Buds responded to the combined effect of auxin and sugar by massive reprogramming of the transcriptome and metabolome. The antagonistic effect of sucrose and auxin targeted several important physiological processes, including sink strength, the amino acid metabolism, the sulfate metabolism, ribosome biogenesis, the nucleic acid metabolism, and phytohormone signaling. Further experiments revealed a role of the TOR-kinase signaling pathway in bud outgrowth through at least downregulation of Rosa hybrida BRANCHED1 (RhBRC1). These new findings represent a cornerstone to further investigate the diverse molecular mechanisms that drive the integration of endogenous factors during shoot branching. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35392520; PUBMED: 35392520; WOS: 000780691900001 |
| DOI: |
10.3389/fpls.2022.830840 |
| Availability: |
https://u-paris.hal.science/hal-03671503; https://u-paris.hal.science/hal-03671503v1/document; https://u-paris.hal.science/hal-03671503v1/file/fpls-13-830840.pdf; https://doi.org/10.3389/fpls.2022.830840 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.30B38EE7 |
| Database: |
BASE |