| Title: |
Binding Mechanism Elucidation of the Acute Respiratory Disease Causing Agent Adenovirus of Serotype 7 to Desmoglein-2 |
| Authors: |
Hograindleur, Marc-André; Effantin, Gregory; Fenel, Daphna; Mas, Caroline; Lieber, André; Schoehn, Guy; Fender, Pascal; Vassal-Stermann, Emilie |
| Contributors: |
Institut de biologie structurale (IBS - UMR 5075); Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG); Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)); Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA); Department of Medicine, Division of Medical Genetics; University of Washington Seattle; ANR-18-CE11-0001,Ad-Cadh,Mécanisme d'interaction des adenovirus du sous-groupe B2 à la cadhérine desmosomale DSG2 et ses applications(2018) |
| Source: |
ISSN: 1999-4915 ; Viruses ; https://hal.univ-grenoble-alpes.fr/hal-02972725 ; Viruses, 2020, 12 (10), pp.1075. ⟨10.3390/v12101075⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2020 |
| Collection: |
Université Grenoble Alpes: HAL |
| Subject Terms: |
acute respiratory distress syndromes; adenoviruses; cryo-electron microscopy; desmoglein-2; virus-host interactions; [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Structural Biology [q-bio.BM] |
| Description: |
International audience ; The study of viruses causing acute respiratory distress syndromes (ARDS) is more essential than ever at a time when a virus can create a global pandemic in a matter of weeks. Among human adenoviruses, adenovirus of serotype 7 (HAdV7) is one of the most virulent serotypes. This virus regularly re-emerges in Asia and has just been the cause of several deaths in the United States. A critical step of the virus life cycle is the attachment of the knob domain of the fiber (HAd7K) to the cellular receptor desmoglein-2 (DSG2). Complexes between the fiber knob and two extracellular domains of DSG2 have been produced. Their characterization by biochemical and biophysical methods show that these two domains are sufficient for the interaction and that the trimeric HAd7K could accommodate up to three DSG2 receptor molecules. The cryo-electron microscopy (cryo-EM) structure of these complexes at 3.1 Å resolution confirmed the biochemical data, and allowed the identification of the critical amino acid residues for this interaction, which shows similarities with other DSG2 interacting adenoviruses, despite a low homology in the primary sequences. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/32992715; PUBMED: 32992715 |
| DOI: |
10.3390/v12101075 |
| Availability: |
https://hal.univ-grenoble-alpes.fr/hal-02972725; https://hal.univ-grenoble-alpes.fr/hal-02972725v1/document; https://hal.univ-grenoble-alpes.fr/hal-02972725v1/file/Hograindleur%20et%20al.%20Viruses.pdf; https://doi.org/10.3390/v12101075 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.30CF6EC2 |
| Database: |
BASE |