| Title: |
Expresión de proteínas inducibles por frío en la médula espinal de rata sometida a hipotermia sistémica |
| Authors: |
Sarotto, Anibal 0000-0002-2199-5524; Rey Funes, Manuel 0000-0002-0213-3056; Dorfman, Verónica 0000-0002-7950-1400; Contartese, Daniela 0000-0003-3690-264X; Larráyoz Roldán, Ignacio Marcos 0000-0003-1629-152X; Martínez Ramírez, Alfredo 0000-0003-4882-4044; Toscanini, Agustina 0000-0001-9431-7794; Loidl, César Fabián 0000-0001-6609-8969 |
| Publisher Information: |
Asociación Argentina de Ortopedia y Traumatología |
| Publication Year: |
2022 |
| Description: |
Introduction: Traumatic spinal cord injury is the main cause of motor disability in developed and underdeveloped countries, being a priority interest to the WHO. The effect of hypothermia on the expression of CIRBP (cold-activated protein) in the anterior grey column of 60-day-old male albino Sprague-Dawley rats was studied at the structural and biochemical levels and proposed as a possible therapeutic approach. Materials and Methods: 24 rats were randomly divided into two groups; normothermia (n = 6), at 24° C, and hypothermia, (n = 18) at 8° C for 180 minutes and euthanized at 12, 24, and 48 h post-treatment. Western blot and immunohistochemistry for CIRBP were used. Results: A progressive increase in the expression of CIRBP was observed from 12 to 48 hours, with statistically significant values after 24 and 48 hours compared to controls. Conclusion: This experimental model demonstrated efficacy, accessibility, and economy to generate systemic hypothermia, which provides a novel range of therapeutic strategies. The increase in the expression of cold-inducible proteins in the rats’ spinal cords allows us to study the benefit of hypo-thermia at the molecular level for the first time, being of utmost importance for therapeutic studies in spinal cord injuries. ; Introducción: La lesión traumática de la médula espinal es la principal causa de discapacidad motora en el mundo, y representa una prioridad para la Organización Mundial de la Salud. Se estudió, a nivel estructural y bioquímico, el efecto de la hipotermia sobre la expresión de la CIRBP (proteína activada por frío) en el asta anterior de la médula de ratas Sprague-Dawley albinas macho de 60 días, planteándola como terapéutica posible. Materiales y Métodos: Se dividió a 24 ratas en dos grupos: normotermia a 24 °C (n = 6) e hipotermia a 8 °C (n = 18), durante 180 min, sacrificadas a las 12, 24 y 48 h después del tratamiento. Se utilizó Western blot e inmunohistoquímica para la CIRBP. Resultados: Se observó un aumento progresivo de la expresión de la CIRBP de ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
Spanish; Castilian |
| ISSN: |
1852-7434 |
| Relation: |
info:eu-repo/semantics/altIdentifier/pissn/1852-7434; Expresión de proteínas inducibles por frío en la médula espinal de rata sometida a hipotermia sistémica, 2022, vol. 87, núm. 3, pág. 393-403; https://investigacion.unirioja.es/documentos/6647a00077099d00ab32d4c6 |
| DOI: |
10.15417/ISSN.1852-7434.2022.87.3.1488 |
| Availability: |
https://investigacion.unirioja.es/documentos/6647a00077099d00ab32d4c6; https://doi.org/10.15417/ISSN.1852-7434.2022.87.3.1488 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.3103D57F |
| Database: |
BASE |