| Title: |
Dietary Supplementation with Boswellia serrata, Verbascum thapsus, and Curcuma longa in Show Jumping Horses: Effects on Serum Proteome, Antioxidant Status, and Anti-Inflammatory Gene Expression |
| Authors: |
Beghelli, Daniela; Zallocco, Lorenzo; Angeloni, Cristina; Bistoni, Onelia; Ronci, Maurizio; Cavallucci, Clarita; Mazzoni, Maria Rosa; Nuccitelli, Anna; Catalano, Chiara; Hrelia, Silvana; Lucacchini, Antonio; Giusti, Laura |
| Contributors: |
Beghelli, Daniela; Zallocco, Lorenzo; Angeloni, Cristina; Bistoni, Onelia; Ronci, Maurizio; Cavallucci, Clarita; Mazzoni, Maria Rosa; Nuccitelli, Anna; Catalano, Chiara; Hrelia, Silvana; Lucacchini, Antonio; Giusti, Laura |
| Publication Year: |
2023 |
| Collection: |
ARUd'A - Archivio Istituzionale della ricerca dell'università Chieti-Pescara (IRIS) |
| Subject Terms: |
Boswellia serrata (Roxb ex Colebr); Curcuma longa; Verbascum thapsu; immune response; inflammation; oxidative stre; serum proteome; sport horses |
| Description: |
Intense exercise can cause inflammation and oxidative stress due to the production of reactive oxygen species. These pathophysiological processes are interdependent, and each one can induce the other, creating a vicious circle. A placebo-controlled blind study was carried out in show jumping horses (n. 16) to evaluate the effects of a commercial dietary supplement (Dolhorse(R) N.B.F. Lanes srl, Milan, Italy) containing Verbascum thapsus leaf powder (1.42%), Curcuma longa (14.280 mg/kg), and Boswellia serrata (Roxb ex Colebr) (14.280 mg/kg) extracts. Before and after 10 days of dietary supplementation, blood samples were collected to evaluate the protein levels, antioxidants, and inflammatory responses by proteomic analysis or real-time Reverse Transcriptase-Polymerase Chain Reaction (real-time RT-PCR). A total of 36 protein spots, connected to 29 proteins, were modulated by dietary supplementation, whereas real-time RT-PCR revealed a significant downregulation of proinflammatory cytokines (interleukin 1 alpha (p < 0.05) and interleukin-6 (0.005), toll-like receptor 4 (p < 0.05), and IKBKB (p < 0.05) in supplemented sport horses. Immunoglobulin chains, gelsolin, plasminogen, vitamin D binding protein, apolipoprotein AIV, and filamin B were overexpressed, whereas haptoglobin, alpha-2-HS-glycoprotein, alpha 2-macroglobulin, afamin, amine oxidase, 60S acidic ribosomal protein, and complement fragments 3, 4, and 7 were reduced. No effect was observed on the antioxidant defense systems. The present results suggest this phytotherapy may reinforce the innate immune responses, thus representing a valid adjuvant to alleviate inflammation, which is a pathophysiological process in sport horses. |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/36983904; info:eu-repo/semantics/altIdentifier/wos/WOS:000959044100001; volume:13; issue:3; firstpage:Art. N° 750; numberofpages:19; journal:LIFE; https://hdl.handle.net/11564/806395 |
| DOI: |
10.3390/life13030750 |
| Availability: |
https://hdl.handle.net/11564/806395; https://doi.org/10.3390/life13030750 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.31924871 |
| Database: |
BASE |