| Title: |
Immunomodulation strategy against Respiratory Syncytial Virus infection by using lung primo-colonizing bacteria |
| Authors: |
Chottin, Claire; Marquant, Quentin; Saint-Criq, Vinciane; Laubreton, Daphne; Drajac, Carole; Bouguyon, Edwige; Remot, Aude; Riffault, Sabine; Thomas, Muriel; Descamps, Delphyne |
| Contributors: |
Virologie et Immunologie Moléculaires (VIM (UR 0892)); Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); MICrobiologie de l'ALImentation au Service de la Santé (MICALIS); AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Infectiologie et Santé Publique (ISP); Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE) |
| Source: |
12th International RSV Symposium (RSV2022); https://hal.inrae.fr/hal-05303039; 12th International RSV Symposium (RSV2022), Sep 2022, Belfast (Northern Ireland), United Kingdom |
| Publisher Information: |
CCSD |
| Publication Year: |
2022 |
| Collection: |
Université de Versailles Saint-Quentin-en-Yvelines: HAL-UVSQ |
| Subject Terms: |
[SDV.IMM]Life Sciences [q-bio]/Immunology; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Subject Geographic: |
Belfast (Northern Ireland); United Kingdom |
| Description: |
International audience ; BackgroundThe respiratory syncytial virus (RSV) is the main etiological agent of bronchiolitis in infants. Few therapeutics areavailable to prevent or treat RSV infection. The neonatal lung environment is responsible for the high susceptibilityof infants to RSV infection. Indeed, at birth the lung is a complex environment characterized by an evolving immunesystem continuously exposed to environmental stimuli including microbiota colonization. We assume that primo-colonizing bacteria are involved in the maturation of lung immunity and thus in the susceptibility of neonates topulmonary infection. The neonatal period constitutes a window of opportunity to immunomodulate lung immuneresponses. The use of bacterial strains during this period could help directing the local immune response towardsa protective immunity to RSV infection. The aim of our project is to demonstrate that primo-colonizing bacterialstrains isolated from neonatal lungs could reduce the severity of RSV pathology.MethodIn a first part, we used neonatal mouse lung explant to i) characterize the immunostimulant property of the primo-colonizing bacterial strains by measuring cytokines secretion (Luminex assays) ii) to evaluate the capacity of thesestrains to interfere with the replication of a recombinant RSV expressing luciferase.Finally, we confirmed the anti-RSV activity of these bacteria by infecting Air-Liquid Interface human cell culture(Mucilair, Epithelix) with a recombinant RSV expressing mCherry.ResultTwenty-five primo-colonizing strains were characterized on neonatal mouse lung explants for their capacity tostimulate cytokine secretion and to interfere with RSV replication. We identified several non-cytotoxic bacterialstrains that could stimulate lung explants for the secretion of type-I cytokine, such as IL-12 and IFNɤ. Thesestrains were also able to reduce RSV viral replication in lung explants. The bacteria 17 was selected for its originalcytokine signature (Type-I immunity and IL-9) in addition with its ... |
| Document Type: |
conference object |
| Language: |
English |
| Availability: |
https://hal.inrae.fr/hal-05303039; https://hal.inrae.fr/hal-05303039v1/document; https://hal.inrae.fr/hal-05303039v1/file/Oral%20Presentations%20at%20RSV2022_abstract%20reference%20order%20copie.pdf |
| Rights: |
https://about.hal.science/hal-authorisation-v1/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.31DC95C7 |
| Database: |
BASE |