| Title: |
Cytotoxic CD8+ T cells and tissue resident memory cells in colorectal cancer based on microsatellite instability and BRAF status |
| Authors: |
Toh, James; Ferguson, Angela L.; Spring, Kevin J. (R17176); Mahajan, Hema; Palendira, Umaimainthan |
| Publisher Information: |
U.S., Baishideng Publishing Group |
| Publication Year: |
2021 |
| Collection: |
University of Western Sydney (UWS): Research Direct |
| Subject Terms: |
XXXXXX - Unknown |
| Description: |
BACKGROUND Recent studies in non-colorectal malignancy have associated T resident memory (T-RM) cells with improved patient survival. It is unknown if T-RM plays a role in colorectal cancer (CRC). AIM To examine the potential role of T-RM cells in providing immunogenicity in CRC stratified by microsatellite instability (MSI) and BRAF status. METHODS Patients with known MSI and BRAF mutation status were eligible for inclusion in this study. CRC tumour sections stained with haematoxylin and eosin were microscopically reviewed and the images scanned prior to assessment for location of invading edge and core of tumour. Sequential sections were prepared for quantitative multiplex immunohistochemistry (IHC) staining. Opal Multiplex IHC staining was performed with appropriate positive and negative controls and imaged using a standard fluorescent microscope fitted with a spectral scanning camera (Mantra) in conjunction with Mantra snap software. Images were unmixed and annotated in inForm 2.2.0. Statistical analysis was performed using Graphpad Prism Version 7 and Stata Version 15. RESULTS Seventy-two patients with known MSI and BRAF status were included in the study. All patients were assessed for MSI by IHC and high resolution capillary electrophoresis testing and 44 of these patients successfully underwent quantitative multiplex IHC staining. Overall, there was a statistically significant increase in CD8+ T-RM cells in the MSI (BRAF mutant and wild type) group over the microsatellite stable (MSS) group. There was a statistically significant difference in CD8+ T-RM between high level MSI (MSI-H):BRAF mutant [22.57, 95% confidence interval (CI): 14.31-30.84] vs MSS [8.031 (95%CI: 4.698-11.36)], P = 0.0076 andMSI-H:BRAF wild type [16.18 (95%CI: 10.44-21.93)] vs MSS [8.031 (95%CI: 4.698-11.36)], P = 0.0279. There was no statistically significant difference in CD8 T cells (both CD8+CD103- and CD8+CD103+T-RM) between MSI-H: BRAF mutant and wild type CRC. CONCLUSION This study has shown that CD8+ T-RM are found in greater ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
print |
| Language: |
English |
| Relation: |
World Journal of Clinical Oncology--2218-4333-- Vol. 12 Issue. 4 No. pp: 238-248 |
| DOI: |
10.5306/wjco.v12.i4.238 |
| Availability: |
https://doi.org/10.5306/wjco.v12.i4.238; https://hdl.handle.net/1959.7/uws:76084 |
| Rights: |
This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) (http://creativecommons.org/Licenses/by-nc/4.0/) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/ |
| Accession Number: |
edsbas.328797B6 |
| Database: |
BASE |