| Title: |
HIV-1 Env induces pexophagy and an oxidative stress leading to uninfected CD4 + T cell death |
| Authors: |
Daussy, Coralie; Galais, Mathilde; Pradel, Baptiste; Robert-Hebmann, Véronique; Sagnier, Sophie; Pattingre, Sophie; Biard-Piechaczyk, Martine; Espert, Lucile |
| Contributors: |
Institut de Recherche en Infectiologie de Montpellier (IRIM); Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS); Institut de Recherche en Cancérologie de Montpellier (IRCM - U1194 Inserm - UM); CRLCC Val d'Aurelle - Paul Lamarque-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM); Université de Montpellier (UM); Centre National de la Recherche Scientifique (CNRS); ANR-10-LABX-0012,EpiGenMed,From Genome and Epigenome to Molecular Medicine: turning new paradigms in biology into the therapeutic strategies of tomorrow(2010) |
| Source: |
ISSN: 1554-8627. |
| Publisher Information: |
CCSD; Taylor & Francis |
| Publication Year: |
2020 |
| Collection: |
Université de Montpellier: HAL |
| Subject Terms: |
peroxisomes; autophagy; apoptosis; ROS; HIV-1 Env; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; The immunodeficiency observed in HIV-1-infected patients is mainly due to uninfected bystander CD4+ T lymphocytes death. The viral envelope glycoproteins (Env), expressed at the surface of infected cells, play a key role in this process. Env triggers autophagy, process necessary to subsequent apoptosis, and to production of Reactive Oxygen Species (ROS) in bystander CD4+ T cells. Here, we demonstrate that Env-induced oxidative stressisresponsible for their death by apoptosis. Moreover, we report that peroxisomes, organelles involved in the control of oxidative stress, are targeted by Env-mediated autophagy. Indeed, we observe a selective autophagy-dependent decrease in the expression of peroxisomal proteins, catalase and PEX14, upon Env exposure, since the down-regulation of either BECLIN 1 or p62/SQSTM1 restores their expression levels. Fluorescence studies allowed us to conclude that Envmediated autophagy degrades these entire organelles and specifically the mature ones.Together, our results on Env-induced pexophagy provide new clues on HIV-1-induced immunodeficiency. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1080/15548627.2020.1831814 |
| Availability: |
https://hal.science/hal-02997474; https://hal.science/hal-02997474v1/document; https://hal.science/hal-02997474v1/file/Autophagy%202020.pdf; https://doi.org/10.1080/15548627.2020.1831814 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.33502CD4 |
| Database: |
BASE |