| Title: |
Listeria tempestatis sp. nov. and Listeria rocourtiae subsp. hofi subsp. nov. |
| Authors: |
Brown, Phillip; Moura, Alexandra; Valès, Guillaume; Tessaud-Rita, Nathalie; Niedermeyer, Jefffrey; Parsons, Cameron; Leclercq, Alexandre; Harris, Angela; Emanuel, Ryan; Kathariou, Sophia; Lecuit, Marc |
| Contributors: |
North Carolina State University Raleigh (NC State); University of North Carolina System (UNC); Centre National de Référence Listeria - National Reference Center Listeria (CNR); Institut Pasteur Paris (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité); Biologie des Infections - Biology of Infection; Université Paris Cité (UPCité); Institut Pasteur Paris (IP); Nicholas School of the Environment; Duke University Durham; Hôpital Necker - Enfants Malades AP-HP; Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP); Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163); Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité); This work was supported by Institut Pasteur, Inserm and Santé Publique France. This work was partially supported by the International Life Sciences Institute (ILSI) North America Food Microbiology Committee. ILSI North America had no role in the design, analysis, interpretation or presentation of the data and results. Partial support was also provided by the College of Agriculture and Life Sciences and the Department of Food, Nutrition and Bioprocessing Sciences at North Carolina State University, Raleigh, NC USA and by award 2018–07464 from the USDA National Institute of Food and Agriculture.; The authors wish to thank the P2M platform (Institut Pasteur, Paris, France) for genome sequencing and the Leibniz Institute DSMZ-German Collection for Microorganisms and Cell Cultures GmbH (Braunschweig, Germany) and the Collection of Institut Pasteur (Paris, France) for depositing the type strains in their collections. |
| Source: |
ISSN: 1466-5026. |
| Publisher Information: |
CCSD; Microbiology Society |
| Publication Year: |
2025 |
| Subject Terms: |
average nucleotide identity (ANI); in silico DNA–DNA hybridization (isDDH); new taxa; whole-genome sequencing; Listeria; Firmicutes; [SDV]Life Sciences [q-bio]; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology |
| Description: |
International audience ; In September 2018, Hurricane Florence resulted in major flooding in North Carolina, USA. Efforts to isolate Listeria monocytogenes and other Listeria spp. from Hurricane Florence floodwaters repeatedly yielded non-haemolytic Listeria -like isolates that could not be readily assigned to known Listeria taxa. Whole-genome sequence analyses against the 28 currently known Listeria species confirmed that the isolates constitute two new taxa within the genus Listeria . Taxon I, with one isolate, showed the highest similarity to Listeria goaensis, with an average nucleotide identity blast of 85.3±4.4% and an in silico DNA–DNA hybridization ( is DDH) of 32.4% (range: 30–35%), differing from the latter by its ability to reduce nitrate, ferment d -ribose and sucrose, and by its inability to produce catalase or ferment d -trehalose and d -lactose. Taxon II, represented by 11 isolates, showed the highest similarity to Listeria rocourtiae, with an average nucleotide identity blast of 92.64±3.8% and an is DDH of 49.9% (range: 47.3–52.5%), differing from the latter by its ability to ferment l -arabinose and its inability to ferment l -rhamnose, d -galactose, d -lactose and d -melibiose. The names Listeria tempestatis sp. nov. and Listeria rocourtiae subsp. hofi subsp. nov. are proposed for taxon I and II, respectively, with type strains CLIP 2022/01175 T (F6L-1A=CIP 112444 T = DSM 117029 T ) and CLIP 2022/01000 T (F66L-1A=CIP 112443 T = DSM 117030 T ), respectively. Both taxa lack known Listeria pathogenic islands, suggesting a lack of pathogenicity for humans. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/40358009; PUBMED: 40358009; PUBMEDCENTRAL: PMC12378429 |
| DOI: |
10.1099/ijsem.0.006774 |
| Availability: |
https://pasteur.hal.science/pasteur-05303919; https://pasteur.hal.science/pasteur-05303919v1/document; https://pasteur.hal.science/pasteur-05303919v1/file/ijsem006774.pdf; https://doi.org/10.1099/ijsem.0.006774 |
| Rights: |
http://creativecommons.org/licenses/by/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.337CD822 |
| Database: |
BASE |