| Title: |
Post-Insemination Infusion of Wharton’s Jelly Mesenchymal Stromal/Stem Cells-Derived Conditioned Medium: A Novel Approach for Improving Pregnancy Outcomes in Problem Mares |
| Authors: |
Del Prete C.; Attolini E.; Merlo B.; Iacono E.; Nocera F. P.; De Martino L.; Longobardi C.; Damiano S.; Longobardi V.; Cocchia N.; Pasolini M. P. |
| Contributors: |
Del Prete, C.; Attolini, E.; Merlo, B.; Iacono, E.; Nocera, F. P.; De Martino, L.; Longobardi, C.; Damiano, S.; Longobardi, V.; Cocchia, N.; Pasolini, M. P. |
| Publication Year: |
2025 |
| Collection: |
IRIS Università degli Studi di Napoli Federico II |
| Subject Terms: |
conditioned medium; endometriti; equine; mesenchymal stromal/stem cell; secretome |
| Description: |
Treating endometritis is one of the major challenges faced by veterinarians. The effect of post-insemination intrauterine infusion Wharton’s jelly mesenchymal stromal/stem cell-derived conditioned medium (WJ-MSC-CM) on the uterine response and pregnancy outcomes in problem mares was evaluated in this study. The cycles of 12 problem mares were treated seven/eight hours after insemination with intrauterine infusion of 20 mL of WJ-MSC-CM (TRT) or non-conditioned Ringer’s lactate solution (CTR). Before (PRE) and 12 h after (POST) treatment, an ultrasonographic examination and a low-volume flush (LVF) for efflux clarity evaluation and bacteriological and cytological analysis were made. Pregnancy diagnosis was performed at 14 days and confirmed at 60 days after ovulation. A decrease (p < 0.05) in polymorphonuclear neutrophils infiltration and a trend toward significance in LVF efflux clarity (p < 0.1) were observed in TRT cycles. The TRT (50%) had a higher pregnancy rate (p < 0.05) than CTR group (0%) at first cycle. Only a trend toward significance was observed comparing pregnancy frequencies between the two groups (CTR 12.5% vs. TRT 54.5%), the pregnancy rate of TRT cycles was comparable to that of normal mares (p > 0.05) and higher (p < 0.05) than that reported for problem mares in the literature. Post-insemination uterine infusion of WJ-MSC-CM in mares restores pregnancy rates to normal levels, by its anti-inflammatory effect. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:001497065200001; volume:12; issue:5; numberofpages:16; journal:VETERINARY SCIENCES; https://hdl.handle.net/11588/1003617 |
| DOI: |
10.3390/vetsci12050482 |
| Availability: |
https://hdl.handle.net/11588/1003617; https://doi.org/10.3390/vetsci12050482 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Dominio pubblico ; license uri:http://creativecommons.org/publicdomain/zero/1.0/ |
| Accession Number: |
edsbas.33ECB801 |
| Database: |
BASE |