| Title: |
Exploring Kafirin's Biochemical and Physicochemical Properties through Size-Exclusion Chromatography ; Exploration des Propriétés Biochimiques et Physicochimiques de Kafirines par Chromatographie d'Exclusion Stérique |
| Authors: |
Knaggs, Freya; Morel, Marie-Hélène; Soldatic, Aleksa; Bonicel, Joëlle; Nigen, Michaël; Sanchez, Christian; Mameri, Hamza |
| Contributors: |
Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier; Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM); International Association for Cereal Science and Technology; INRAE |
| Source: |
17. International Cereal and Bread Congress (ICBC24); https://hal.inrae.fr/hal-05207455; 17. International Cereal and Bread Congress (ICBC24), International Association for Cereal Science and Technology; INRAE, Apr 2024, Nantes, France |
| Publisher Information: |
CCSD |
| Publication Year: |
2024 |
| Collection: |
Université de Montpellier: HAL |
| Subject Terms: |
Sorghum; Kafirin; Experimental design; Extraction; Plan d'expérience; Sorgho; [SDV.IDA]Life Sciences [q-bio]/Food engineering; [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition; [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry; Molecular Biology/Biochemistry [q-bio.BM] |
| Subject Geographic: |
Nantes; France |
| Description: |
International audience ; Sorghum, the fifth most produced and consumed cereal crop globally, is a rich source of macro and micronutrients. Its grain contains 7-14% proteins, predominantly kafirins. Compared to other cereal grain storage proteins, kafirins are more hydrophobic and highly crosslinked. In consequence they present higher resistance to hydrolysis by gastrointestinal proteases, and so a lower digestibility. This work aims to develop a reliable kafirin extraction protocol in view of their exhaustive biochemical and physicochemical characterization.We investigated the impact of various factors, such as grain dehulling, extracting solvent type, extraction time, sample to liquid ratio and temperature on the yield of kafirin extraction. Using an experimental design approach with two to three levels for factors, we employed size-exclusion chromatography (SE-HPLC) with UV detection to analyse the composition of the protein extracts. We determined the kafirin conversion coefficient at 214 using the Kjeldahl method as a reference to calculate the extraction yield from the SEC profiles. Additionally, kafirin composition was assessed through SDS-PAGE in reduced and non-reduced conditions .Our key finding was that initial grain dehulling, a 60% tert-butanol - water (v/v) solvent, a temperature of 60°C, a sample-to-solvent ratio of 1:100, and two extraction cycles of 10 minutes resulted in the maximum extraction yield. The extraction parameters significantly influenced the extraction of kafirins, offering insights into the development of a robust extraction protocol.While various extraction procedures have been reported in the literature, they are mainly based on the use of alkali and sodium bisulfite leading to kafirin denaturation. Therefore, the fractioning and characterisation of kafirins in their native state remain limited. This study contributes to the understanding of kafirin's solubility properties and open the way for their future fractionation in native state. From that the determination of the ... |
| Document Type: |
conference object |
| Language: |
English |
| Availability: |
https://hal.inrae.fr/hal-05207455; https://hal.inrae.fr/hal-05207455v1/document; https://hal.inrae.fr/hal-05207455v1/file/Abstract%20ICBC%202024.pdf |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.3408BA24 |
| Database: |
BASE |