| Title: |
Verubulin (Azixa) Analogues with Increased Saturation: Synthesis, SAR and Encapsulation in Biocompatible Nanocontainers Based on Ca 2+ or Mg 2+ Cross-Linked Alginate |
| Authors: |
Kseniya N. Sedenkova; Denis N. Leschukov; Yuri K. Grishin; Nikolay A. Zefirov; Yulia A. Gracheva; Dmitry A. Skvortsov; Yanislav S. Hrytseniuk; Lilja A. Vasilyeva; Elena A. Spirkova; Pavel N. Shevtsov; Elena F. Shevtsova; Alina R. Lukmanova; Vasily V. Spiridonov; Alina A. Markova; Minh T. Nguyen; Alexander A. Shtil; Olga N. Zefirova; Alexander A. Yaroslavov; Elena R. Milaeva; Elena B. Averina |
| Source: |
Pharmaceuticals, Vol 16, Iss 1499, p 1499 (2023) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2023 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
tubulin polymerization inhibitor; apoptosis inducer; verubulin; pyrimidine; tetrahydroquinazoline; aromatic nucleophilic substitution; Medicine; Pharmacy and materia medica; RS1-441 |
| Description: |
Tubulin-targeting agents attract undiminished attention as promising compounds for the design of anti-cancer drugs. Verubulin is a potent tubulin polymerization inhibitor, binding to colchicine-binding sites. In the present work, a series of verubulin analogues containing a cyclohexane or cycloheptane ring 1,2-annulated with pyrimidine moiety and various substituents in positions 2 and 4 of pyrimidine were obtained and their cytotoxicity towards cancer and non-cancerous cell lines was estimated. The investigated compounds revealed activity against various cancer cell lines with IC 50 down to 1–4 nM. According to fluorescent microscopy data, compounds that showed cytotoxicity in the MTT test disrupt the normal cytoskeleton of the cell in a pattern similar to that for combretastatin A-4. The hit compound ( N -(4-methoxyphenyl)- N ,2-dimethyl-5,6,7,8-tetrahydroquinazolin-4-amine) was encapsulated in biocompatible nanocontainers based on Ca 2+ or Mg 2+ cross-linked alginate and it was demonstrated that its cytotoxic activity was preserved after encapsulation. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1424-8247/16/10/1499; https://doaj.org/toc/1424-8247; https://doaj.org/article/d15585dc1f164f22bf292e2c887d7428 |
| DOI: |
10.3390/ph16101499 |
| Availability: |
https://doi.org/10.3390/ph16101499; https://doaj.org/article/d15585dc1f164f22bf292e2c887d7428 |
| Accession Number: |
edsbas.34A47BF2 |
| Database: |
BASE |