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Microbial Growth and Endotoxin Production in the Intravenous Anesthetic Propofol

Title: Microbial Growth and Endotoxin Production in the Intravenous Anesthetic Propofol
Authors: Arduino, Matthew J.; Bland, Lee A.; McAllister, Sigrid K.; Aguero, Sonia M.; Villarino, Margarita E.; McNeil, Michael M.; Jarvis, William R.; Favero, Martin S.
Source: Infection Control & Hospital Epidemiology ; volume 12, issue 9, page 535-539 ; ISSN 0899-823X 1559-6834
Publisher Information: Cambridge University Press (CUP)
Publication Year: 1991
Description: Objective: In this study, we measured microbial growth and endotoxin production in the intravenous anesthetic propofol using 10 different microbial strains; 6 isolated from outbreak cases and 4 from laboratory stock cultures. Design: In each trial, endotoxin-free glass tubes containing 10 ml propofol were inoculated with 10°-10 3 CFU/ml of the test organism and incubated at 30°C for 72 hours. Setting: In May and June 1990, the Centers for Disease Control received reports of 5 outbreaks in 5 states of postsurgical patient infections and/or pyrogenic reactions. Epidemiologic and laboratory investigations implicated extrinsic contamination of an intravenous anesthetic, propofol, as the probable source of these outbreaks. Results: After 24 hours, 9 of the 10 cultures increased in viable counts by 3 to 6 logs. At least 1 ng/ml of endotoxin was produced within 24 hours by Escherichia coli, Enterobacter cloacae, and Acinetobacter calcoaceticus subspecies anitratus. Conclusions: Propofol can support rapid microbial growth and endotoxin production. To avoid infectious complications, scrupulous aseptic technique should be used when preparing or administering this anesthetic.
Document Type: article in journal/newspaper
Language: English
DOI: 10.1086/646405
Availability: https://doi.org/10.1086/646405; https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0899823X00086098
Rights: https://www.cambridge.org/core/terms
Accession Number: edsbas.34C99C8D
Database: BASE