| Title: |
Ex Vivo Host Transcriptomics During Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans Infection of Peripheral Blood Mononuclear Cells From South African Volunteers. |
| Authors: |
Doyle, RM; Kannambath, S; Pittman, A; Goliath, R; Kumar, V; Meintjes, G; Milburn, J; Netea, MG; Harrison, TS; Jarvis, JN; Bicanic, T |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2025 |
| Collection: |
St George's University of London: Repository |
| Description: |
Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans are opportunistic fungal pathogens associated with infections in immunocompromised hosts. Cryptococcal meningitis (CM) is the leading fungal cause of human immunodeficiency virus-related deaths globally, with the majority occurring in Africa. The human immune response to C albicans infection has been studied extensively in large genomics studies whereas cryptococcal infections, despite their severity, are comparatively understudied. Here we investigated the transcriptional response of immune cells after in vitro stimulation with in vitro C neoformans, C gattii, and C albicans infection of peripheral blood mononuclear cells collected from healthy South African volunteers. We found a lower transcriptional response to cryptococcal stimuli compared to C albicans and unique expression signatures from all 3 fungal stimuli. This work provides a starting point for further studies comparing the transcriptional signature of CM in immunocompromised patients, with the goal of identifying biomarkers of disease severity and possible novel treatment targets. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf; application/zip |
| Language: |
English |
| ISSN: |
1537-6613 |
| Relation: |
https://openaccess.sgul.ac.uk/id/eprint/116767/9/jiae410.pdf; https://openaccess.sgul.ac.uk/id/eprint/116767/8/jiae410_supplementary_data.zip; https://openaccess.sgul.ac.uk/id/eprint/116767/1/accepted_jiae410.pdf; Doyle, RM; Kannambath, S; Pittman, A; Goliath, R; Kumar, V; Meintjes, G; Milburn, J; Netea, MG; Harrison, TS; Jarvis, JN; et al. Doyle, RM; Kannambath, S; Pittman, A; Goliath, R; Kumar, V; Meintjes, G; Milburn, J; Netea, MG; Harrison, TS; Jarvis, JN; Bicanic, T (2025) Ex Vivo Host Transcriptomics During Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans Infection of Peripheral Blood Mononuclear Cells From South African Volunteers. J Infect Dis, 231 (1). e254-e262. ISSN 1537-6613 https://doi.org/10.1093/infdis/jiae410 SGUL Authors: Pittman, Alan Michael Bicanic, Tihana Harrison, Thomas Stephen |
| DOI: |
10.1093/infdis/jiae410 |
| Availability: |
https://openaccess.sgul.ac.uk/id/eprint/116767/; https://openaccess.sgul.ac.uk/id/eprint/116767/9/jiae410.pdf; https://openaccess.sgul.ac.uk/id/eprint/116767/8/jiae410_supplementary_data.zip; https://openaccess.sgul.ac.uk/id/eprint/116767/1/accepted_jiae410.pdf; https://doi.org/10.1093/infdis/jiae410 |
| Rights: |
cc_by_4 |
| Accession Number: |
edsbas.353E521F |
| Database: |
BASE |