| Title: |
Fine mapping and transcriptomics reveal OSG function in regulation of grain size and pollen fertility in rice (Oryza sativa) |
| Authors: |
Zhao, Sijie; Zhang, Siyue; Xu, Weichen; Fang, Sijia; Liu, Mei; Wang, Lei; Zhang, Xianyang; Chen, Bingxu; Wei, Shuya; Zhao, Heming |
| Contributors: |
Rashid, Muhammad Abdul Rehman; the Natural Science Foundation of Anhui Province; the key project of the Education Department of Anhui Province; the Excellent Scientific Research and Innovation team of the Education Department of Anhui Province; the Key Discipline Construction Fund for Crop Science of Anhui Science and Technology University; the Scientific and Technological Innovation Team of Anhui University of Science and Technology; the Talent Introduction Start-up Fund Project of Anhui University of Science and Technology; the Hubei Provincinal Natural Science Foundation-General Project; National College Students Innovation and Entrepreneurship Training Program; College Student Innovation and Entrepreneurship Training Program Project of Anhui Province |
| Source: |
PLOS One ; volume 21, issue 1, page e0338401 ; ISSN 1932-6203 |
| Publisher Information: |
Public Library of Science (PLoS) |
| Publication Year: |
2026 |
| Collection: |
PLOS Publications (via CrossRef) |
| Description: |
Grain shape is a critical factor that directly influences rice yield and quality, however, the molecular mechanisms underlying the regulation of grain shape development remains elusive. In this study, we characterized an oval-shaped grain mutant, osg , from the rice radiation mutagenesis mutant library via 60 Co-γ ray irradiation. Compared to ZH11, the osg mutant exhibited decreased grain length and thousand-grain weight but increased grain width and thickness, while displaying significantly reduced plant height, tiller number, pollen viability, and seed setting rate. Map-based cloning revealed that OsSRS3 , a known regulator of rice grain size and fertility, was identified as the candidate gene for OSG . The loss-of-function mutant in OsSRS3 exhibit abnormal phenotypes similar to that in osg . Comparative transcriptome sequencing of young panicles from ZH11 and osg showed that up-regulated genes were predominantly enriched in pathways related to plant hormone signal transduction and MAPK signaling, whereas down-regulated genes were mostly associated with starch and sucrose metabolism. Further analysis of differentially expressed genes (DEGs) within the pathways revealed that multiple DEGs in osg , such as OsJAZ11 , OsUgp1 and OsUgp2 , have been functionally characterized, and their mutant phenotypes align with those observed in osg . These findings provide a crucial foundation for elucidating the molecular mechanisms by which the SRS3 gene regulates grain size and fertility in rice. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1371/journal.pone.0338401 |
| Availability: |
https://doi.org/10.1371/journal.pone.0338401; https://dx.plos.org/10.1371/journal.pone.0338401 |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.3696AFFF |
| Database: |
BASE |