| Title: |
Helminth extracellular vesicles co‐opt host monocytes to drive T cell anergy |
| Authors: |
Borup, Anne; Sharifpour, Mohammad Farouq; Rossen, Litten S.; Whitehead, Bradley; Boysen, Anders T.; Olesen, Rikke; Bohn, Anja B.; Ridolfi, Andrea; Brucale, Marco; Valle, Francesco; Paolini, Lucia; Radeghieri, Annalisa; Bergese, Paolo; Miles, Kim; Veitch, Margaret; Thomas, Tamara; Ruscher, Roland; Wangchuk, Phurpa; Giacomin, Paul; Loukas, Alex; Nejsum, Peter |
| Contributors: |
Borup Anne; Sharifpour Mohammad Farouq; Rossen Litten S.; Whitehead Bradley; Boysen Anders T.; Olesen Rikke; Bohn Anja B.; Ridolfi Andrea; Brucale Marco; Valle Francesco; Paolini Lucia; Radeghieri Annalisa; Bergese Paolo; Miles Kim; Veitch Margaret; Thomas Tamara; Ruscher Roland; Wangchuk Phurpa; Giacomin Paul; Loukas Alex; Nejsum Peter |
| Publication Year: |
2025 |
| Collection: |
Università degli Studi di Brescia: OPENBS - Open Archive UniBS |
| Subject Terms: |
Ascari; coliti; extracellular vesicle; helminth; host‐parasite interaction; immune modulation; inflammatory bowel disease; monocytes |
| Description: |
Parasitic helminths secrete extracellular vesicles (EVs) into their host tissues to modulate immune responses, but the underlying mechanisms are poorly understood. We demonstrate that Ascaris EVs are efficiently internalised by monocytes in human peripheral blood mononuclear cells and increase the percentage of classical monocytes. Furthermore, EV treatment of monocytes induced a novel anti-inflammatory phenotype characterised by CD14+, CD16−, CC chemokine receptor 2 (CCR2−) and programmed death-ligand 1 (PD-L1)+ cells. In addition, Ascaris EVs induced T cell anergy in a monocyte-dependent mechanism. Targeting professional phagocytes to induce both direct and indirect pathways of immune modulation presents a highly novel and efficient mechanism of EV-mediated host-parasite communication. Intra-peritoneal administration of EVs induced protection against gut inflammation in the dextran sodium sulphate model of colitis in mice. Ascaris EVs were shown to affect circulating immune cells and protect against gut inflammation; this highlights their potential as a subject for further investigation in inflammatory conditions driven by dysregulated immune responses. However, their clinical translation would require further studies and careful consideration of ethical implications. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/39815783; info:eu-repo/semantics/altIdentifier/wos/WOS:001396495900001; volume:14; issue:1; journal:JOURNAL OF EXTRACELLULAR VESICLES; https://hdl.handle.net/11379/633026 |
| DOI: |
10.1002/jev2.70027 |
| Availability: |
https://hdl.handle.net/11379/633026; https://doi.org/10.1002/jev2.70027 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:PUBBLICO - Creative Commons 4.0 ; license uri:iris.PUB15 |
| Accession Number: |
edsbas.38D9F1B5 |
| Database: |
BASE |