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DNA-PKcs is required for cGAS/STING-dependent viral DNA sensing in human cells.

Title: DNA-PKcs is required for cGAS/STING-dependent viral DNA sensing in human cells.
Authors: Hristova, DB; Oliveira, M; Wagner, E; Melcher, A; Harrington, KJ; Belot, A; Ferguson, BJ
Contributors: Melcher, Alan; Harrington, Kevin
Publisher Information: CELL PRESS
Publication Year: 2024
Collection: The Institute of Cancer Research (ICR): Publications Repository
Subject Terms: Molecular biology; Virology
Subject Geographic: United States
Description: To mount an efficient interferon response to virus infection, intracellular pattern recognition receptors (PRRs) sense viral nucleic acids and activate anti-viral gene transcription. The mechanisms by which intracellular DNA and DNA viruses are sensed are relevant not only to anti-viral innate immunity, but also to autoinflammation and anti-tumour immunity through the initiation of sterile inflammation by self-DNA recognition. The PRRs that directly sense and respond to viral or damaged self-DNA function by signaling to activate interferon regulatory factor (IRF)-dependent type one interferon (IFN-I) transcription. We and others have previously defined DNA-dependent protein kinase (DNA-PK) as an essential component of the DNA-dependent anti-viral innate immune system. Here, we show that DNA-PK is essential for cyclic GMP-AMP synthase (cGAS)- and stimulator of interferon genes (STING)-dependent IFN-I responses in human cells during stimulation with exogenous DNA and infection with DNA viruses.
Document Type: article in journal/newspaper
File Description: Electronic-eCollection; application/pdf
Language: English
ISSN: 2589-0042
Relation: 108760; S2589-0042(23)02837-7; iScience, 2024, 27 (1), pp. 108760 -; https://repository.icr.ac.uk/handle/internal/6187
DOI: 10.1016/j.isci.2023.108760
Availability: https://doi.org/10.1016/j.isci.2023.108760; https://repository.icr.ac.uk/handle/internal/6187
Rights: http://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.3A0C0DC2
Database: BASE