| Description: |
Figure 1: Experimental workflow. Gametes of Ulva mutabilis were gathered and separated from bacteria (Califano and Wichard 2018). These axenic gametes were cultivated for six weeks to a callus stage. Afterwards, the stock cultures of calli were unified, mixed and distributed in six culture flasks under sterile conditions. One biological triplicate served as control (in 100 % UCM), while the cultures for the AGMPF-treated biological triplicate [in sterile-filtered Roseovarius UCM-based supernatant (75 %) and UCM (25 %); v:v] were inoculated subsequently with (−)-thallusin (2 × 10−8 mol l−1 final concentration). The calli were incubated for two weeks, separated from the culture medium for solvent extraction, and flash-frozen. After further sample preparation, the ground calli were extracted using a commercial kit based on the Folch lipid extraction method (Folch et al. 1957). In the ensuing sample processing, one aliquot of each sample extract was subjected to a UHPLC-MS Orbitrap system to conduct endo-metabolomics. The data were analysed and evaluated using Compound Discoverer (vers. 3.3) and Metaboanalyst (vers. 6.0). Fatty acids were derivatised to obtain methyl esters for quantification by GC-EI-MS. The analysed fatty acids were quantified based on their response factor through a comparison with an external standard mix. For detailed information, see Supplementary Material. ; Published as part of Holbl, Short Communication Hermann, Dunger, Nico & Wichard, Thomas, 2025, Bacteria-released algal growth and morphogenesis factors regenerate axenic calli derived from the macroalga Ulva (Chlorophyta) and change the fatty acid profile, pp. 193-200 in Botanica Marina (Warsaw, Poland) 68 (3) on page 194, DOI:10.1515/bot-2024-0101, http://zenodo.org/record/16957352 |