| Title: |
Identification of Two Eosinophil Subsets in Induced Sputum from Patients with Allergic Asthma According to CD15 and CD66b Expression |
| Authors: |
Curto Sánchez, Elena; Mateus-Medina, Éder Fredy; Crespo Lessmann, Astrid; Osuna Gómez, Rubén; Ujaldón Miró, Cristina; García-Moral, Alba; Galvan-Blasco, Paula; Soto-Retes, Lorena; Ramos-Barbón, David; Plaza, Vicente; Universitat Autònoma de Barcelona |
| Publication Year: |
2022 |
| Collection: |
Universitat Autònoma de Barcelona: Dipòsit Digital de Documents de la UAB |
| Subject Terms: |
Allergic asthma; Eosinophil subsets; Flow cytometry; Induced sputum |
| Description: |
Altres ajuts: Spanish Allergy and Clinical Immunology Society (SEAIC) (reference 17/06); BRN-Fundació Pla i Armengol grant in the call of 2018. ; Two subsets of eosinophils have been described: resident eosinophils with homeostatic functions (rEOS) in healthy subjects and in patients with nonallergic eosinophilic asthma, and inflammatory eosinophils (iEOS) in blood and lung samples from patients with allergic asthma. We explored if it would be possible to identify different subsets of eosinophils using flow cytometry and the gating strategy applied to induced sputum. We conducted an observational cross-sectional single-center study of 62 patients with persistent allergic asthma. Inflammatory cells from induced sputum samples were counted by light microscopy and flow cytometry, and cytokine levels in the supernatant were determined. Two subsets of eosinophils were defined that we call E1 (CD66b-high and CD15-high) and E2 (CD66b-low and CD15-low). Of the 62 patients, 24 were eosinophilic, 18 mixed, 10 paucigranulocytic, and 10 neutrophilic. E1 predominated over E2 in the eosinophilic and mixed patients (20.86% vs. 6.27% and 14.42% vs. 4.31%, respectively), while E1 and E2 were similar for neutrophilic and paucigranulocytic patients. E1 correlated with IL-5, fractional exhaled nitric oxide, and blood eosinophils. While eosinophil subsets have been identified for asthma in blood, we have shown that they can also be identified in induced sputum. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
16604601 |
| Relation: |
International journal of environmental research and public health; Vol. 19 Núm. 20 (october 2022), p. 13400; https://ddd.uab.cat/record/277694; urn:10.3390/ijerph192013400; urn:oai:ddd.uab.cat:277694; urn:scopus_id:85140921880; urn:pmid:36293979; urn:articleid:16604601v19n20p13400; urn:oai:pubmedcentral.nih.gov:9602830; urn:pmcid:PMC9602830 |
| Availability: |
https://ddd.uab.cat/record/277694 |
| Rights: |
open access ; Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.3D613EB2 |
| Database: |
BASE |