The impact of viral mutations on recognition by SARS-CoV-2 specific T cells
| Title: | The impact of viral mutations on recognition by SARS-CoV-2 specific T cells |
|---|---|
| Authors: | TI de Silva; G Liu; BB Lindsey; D Dong; SC Moore; NS Hsu; D Shah; D Wellington; AJ Mentzer; A Angyal; R Brown; MD Parker; Z Ying; X Yao; L Turtle; S Dunachie; DM Aanensen; K Abudahab; H Adams; A Adams; S Afifi; D Aggarwal; SSY Ahmad; L Aigrain; A Alcolea-Medina; NF Alikhan; E Allara; R Amato; T Annett; S Aplin; CV Ariani; H Asad; A Ash; P Ashfield; F Ashford; L Atkinson; SW Attwood; C Auckland; A Aydin; DJ Baker; P Baker; CE Balcazar; J Ball; JC Barrett; M Barrow; E Barton; M Bashton; AR Bassett; R Batra; C Baxter; N Bayzid; C Beaver; AH Beckett; SM Beckwith; L Bedford; R Beer; A Beggs; KL Bellis; L Berry; B Bertolusso; A Best; E Betteridge; D Bibby; K Bicknell; D Binns; A Birchley; PW Bird; C Bishop; R Blacow; V Blakey; B Blane; F Bolt; J Bonfield; S Bonner; D Bonsall; T Boswell; A Bosworth; Y Bourgeois; O Boyd; DT Bradley; C Breen; C Bresner; J Breuer; S Bridgett; IF Bronner; E Brooks; A Broos; JR Brown; G Bucca; SL Buchan; D Buck; M Bull; PJ Burns; S Burton-Fanning; T Byaruhanga; M Byott; S Campbell; AM Carabelli; JS Cargill; M Carlile |
| Publication Year: | 2021 |
| Collection: | University of Sussex (US): Figshare |
| Subject Terms: | Immune response; Immunology; Molecular biology; Phylogenetics; Virology; COVID-19 Genomics UK (COG-UK) Consortium; ISARIC4C Investigators; Biodefense; Emerging Infectious Diseases; Clinical Research; Immunization; Infectious Diseases; Vaccine Related; Prevention; 3 Good Health and Well Being |
| Description: | We identify amino acid variants within dominant SARS-CoV-2 T cell epitopes by interrogating global sequence data. Several variants within nucleocapsid and ORF3a epitopes have arisen independently in multiple lineages and result in loss of recognition by epitope-specific T cells assessed by IFN-γ and cytotoxic killing assays. Complete loss of T cell responsiveness was seen due to Q213K in the A∗01:01-restricted CD8+ ORF3a epitope FTSDYYQLY207-215; due to P13L, P13S, and P13T in the B∗27:05-restricted CD8+ nucleocapsid epitope QRNAPRITF9-17; and due to T362I and P365S in the A∗03:01/A∗11:01-restricted CD8+ nucleocapsid epitope KTFPPTEPK361-369. CD8+ T cell lines unable to recognize variant epitopes have diverse T cell receptor repertoires. These data demonstrate the potential for T cell evasion and highlight the need for ongoing surveillance for variants capable of escaping T cell as well as humoral immunity. |
| Document Type: | article in journal/newspaper |
| Language: | unknown |
| Relation: | 10779/uos.23815566.v1 |
| Availability: | https://figshare.com/articles/journal_contribution/The_impact_of_viral_mutations_on_recognition_by_SARS-CoV-2_specific_T_cells/23815566 |
| Rights: | CC BY 4.0 |
| Accession Number: | edsbas.3D9ADBB6 |
| Database: | BASE |