| Title: |
Noninvasive characterization of oocyte deformability in microconstrictions |
| Authors: |
Barbier, Lucie; Bulteau, Rose; Rezaei, Behnam; Panier, Thomas; Letort, Gaëlle; Labrune, Elsa; Verlhac, Marie-Hélène; Vernerey, Franck; Campillo, Clément; Terret, Marie-Emilie |
| Contributors: |
Centre interdisciplinaire de recherche en biologie (CIRB); Labex MemoLife; École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Collège de France (CdF (institution))-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris); Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL); Université Paris Sciences et Lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Laboratoire Analyse, Modélisation et Matériaux pour la Biologie et l'Environnement (LAMBE - UMR8587); Université d'Évry-Val-d'Essonne (UEVE)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-CY Cergy Paris Université (CY); University of Colorado Boulder; Laboratoire Jean Perrin (LJP); Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS); Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS); Biologie du Développement et Cellules souches (Département) (CNRS UMR3738); Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité); Institut cellule souche et cerveau / Stem Cell and Brain Research Institute (SBRI); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Institut universitaire de France (IUF); Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.); The Verlhac-Terret laboratory is supported by CNRS, INSERM, Collège de France, and the Bettencourt Schueller Foundation. This work received support under the program “Investissements d’Avenir,” launched by the French Government and implemented by the ANR, with the references: ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001-02 PSL* Research University, and through grants from the Fondation pour la Recherche Médicale (FRM label DEQ201903007796 to M.-H.V.), the ANR (ANR-18-CE13 and ANR-22-CE13 to M.-H.V. and ANR-24-CE13-5691 to M.-E.T.), DIM ELICIT du Conseil regional d’Ile de France (DIM ELICIT-AAP-2020 to M.-E.T.), Biomedical Engineering seed grant program (BME to C.C.), PSL-QLife interdisciplinary program (QLife-CdF-06-2022 to M.-E.T.), FRM ECO-Contrat doctoral program (ECO202206015524 to R.B.), and U.S National Science Foundation (award abstract no. 2135057, Collaborative Research: RECODE: Organoid model of growth plate development to F.V.).; ANR-10-LABX-0054,MEMOLIFE,Memory in living systems: an integrated approach(2010); ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011); ANR-24-CE13-5691,CoPOcyte,Tension corticale, polarisation et potentiel de développement des ovocytes de mammifères(2024) |
| Source: |
ISSN: 2375-2548 ; Science Advances ; https://hal.science/hal-04964020 ; Science Advances , 2025, 11 (8), pp.eadr9869. ⟨10.1126/sciadv.adr9869⟩. |
| Publisher Information: |
CCSD; American Association for the Advancement of Science (AAAS) |
| Publication Year: |
2025 |
| Collection: |
Inserm: HAL (Institut national de la santé et de la recherche médicale) |
| Subject Terms: |
[PHYS.MECA.BIOM]Physics [physics]/Mechanics [physics]/Biomechanics [physics.med-ph]; [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft]; [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] |
| Description: |
L.B., R.B., M.-H.V., C.C., and M.-E.T. are inventors on patent application EP23166408.7 submitted by Université PSL that covers “microfluidic device for use in a system for measuring at least one biomarker of at least one oocyte.” The other authors declare that they have no competing interests. ; International audience ; Oocytes naturally present mechanical defects that hinder their development after fertilization. Thus, in the context of assisted reproduction, oocyte selection based on their mechanical properties has great potential to improve the quality of the resulting embryos and the success rate of these procedures. However, using mechanical properties as a quantifiable selective criterion requires robust and nondestructive measurement tools. This study developed a constriction-based microfluidic device that monitors the deformation of mouse oocytes under controlled pressure. The device can distinguish mechanically aberrant oocyte groups from healthy control ones. On the basis of a mathematical model, we propose that deformability measurements infer both oocyte tension and elasticity, elasticity being the most discriminating factor in our geometry. Despite force transmission during oocyte deformation, no long-term damage was observed. This noninvasive characterization of mouse oocyte deformability in microconstrictions allows for a substantial advance in assessing the mechanical properties of mammalian oocytes and has potential application as a quantifiable selective criterion in medically assisted reproduction. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://doi.org/10.5281/zenodo.14199390; info:eu-repo/semantics/altIdentifier/pmid/39970229; PUBMED: 39970229; PUBMEDCENTRAL: PMC11838009 |
| DOI: |
10.1126/sciadv.adr9869 |
| Availability: |
https://hal.science/hal-04964020; https://hal.science/hal-04964020v1/document; https://hal.science/hal-04964020v1/file/Barbier%202025.pdf; https://doi.org/10.1126/sciadv.adr9869 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.3DADA5E6 |
| Database: |
BASE |